IDENTIFICATION AND SEQUENCE DETERMINATION OF THE HOST FACTOR GENE FOR BACTERIOPHAGE-Q-BETA

被引：103

作者：

KAJITANI, M

ISHIHAMA, A

机构：

[1] NATL INST GENET,DEPT MOLEC GENET,MISHIMA,SHIZUOKA 411,JAPAN

[2] TORAY INDUSTRIES LTD,BASIC RES LABS,KAMAKURA,KANAGAWA 248,JAPAN

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 05期

关键词：

D O I：

10.1093/nar/19.5.1063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The host factor (HF-I) required for phage Q-beta RNA-directed synthesis of complementary minus-strand RNA was purified to homogeneity from phage-infected Escherichia coli cells. The hfq gene encoding HF-I was cloned using synthetic probes designed based on the partial amino acid sequence of HF-I, and mapped at 94.8 min on the E. coli chromosome downstream of the miaA gene involved in 2-methylthio-N6-(isopentyl)-adenosine (ms2i6A) tRNA modification. Sequence determination of the cloned hfq gene indicated that HF-I is a small protein of Mr 11,166 consisting of 102 amino acid residues.

引用

页码：1063 / 1066

页数：4

共 20 条

[1] IDENTIFICATION OF POLYPEPTIDES ENCODED BY AN ESCHERICHIA-COLI LOCUS (HFLA) THAT GOVERNS THE LYSIS-LYSOGENY DECISION OF BACTERIOPHAGE-LAMBDA [J].