FREE-RADICAL REACTIONS WITH PROTEINS AND ENZYMES - INACTIVATION OF PEPSIN

被引：20
作者：
ADAMS, GE
POSENER, ML
BISBY, RH
CUNDALL, RB
KEY, JR
机构：
[1] MT VERNON HOSP,CANC RES CAMPAIGN,GRAY LAB,NORTHWOOD HA6 2RN,MIDDLESEX,ENGLAND
[2] UNIV NOTTINGHAM,DEPT CHEM,NOTTINGHAM NG7 2RD,ENGLAND
来源：
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY | 1979年 / 35卷 / 06期
关键词：
D O I：
10.1080/09553007914550611
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The reactions of free radicals produced by ionizing radiation with pepsin have been studied by steady-state inactivation measurements and by pulse radiolysis. In de-aerated solutions the hydroxyl radical has been found to be the most efficient of the primary free radicals generated from water in causing inactivation. The reactions of the more selective oxidizing inorganic radical anions Br and (SCN), with pepsin have also been examined. In the case of the thiocyanate radical anion (SCN), the inactivation efficiency is found to depend on SCN- concentration, an effect shown to arise from a reversible redox reaction involving the tryptophan and (SCN) radicals. The results demonstrate that tryptophan residue plays an essential role in the enzymic activity of pepsin. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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页码：497 / 507
页数：11
相关论文
共 21 条
[1]   MECHANISM OF RADIATION-INDUCED INACTIVATION OF RIBONUCLEASE IN DILUTE AQUEOUS SOLUTION [J].
ADAMS, GE ;
WILLSON, RL ;
BISBY, RH ;
CUNDALL, RB .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY AND RELATED STUDIES IN PHYSICS CHEMISTRY AND MEDICINE, 1971, 20 (05) :405-&
[2]   SELECTIVE FREE-RADICAL REACTIONS WITH PROTEINS AND ENZYMES - REACTIONS OF INORGANIC RADICAL ANIONS WITH AMINO-ACIDS [J].
ADAMS, GE ;
ALDRICH, JE ;
BISBY, RH ;
WILLSON, RL ;
CUNDALL, RB ;
REDPATH, JL .
RADIATION RESEARCH, 1972, 49 (02) :278-&
[3]   SPECTROSCOPIC STUDIES OF REACTIONS OF OH RADICAL IN AQUEOUS SOLUTIONS [J].
ADAMS, GE ;
BOAG, JW ;
MICHAEL, BD .
TRANSACTIONS OF THE FARADAY SOCIETY, 1965, 61 (507P) :492-&
[4]  
ADAMS GE, 1973, J CHEM SOC F1, V69, P1608
[5]   The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin [J].
Anson, ML .
JOURNAL OF GENERAL PHYSIOLOGY, 1938, 22 (01) :79-89
[6]   PULSE RADIOLYSIS OF AQUEOUS THIOCYANATE AND IODIDE SOLUTIONS [J].
BAXENDALE, JH ;
BEVAN, PLT ;
STOTT, DA .
TRANSACTIONS OF THE FARADAY SOCIETY, 1968, 64 (549P) :2389-+
[7]   ONE-ELECTRON REDUCTION REACTIONS WITH ENZYMES IN SOLUTION - PULSE-RADIOLYSIS STUDY [J].
BISBY, RH ;
CUNDALL, RB ;
REDPATH, JL ;
ADAMS, GE .
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1976, 72 :51-63
[8]   SELECTIVE FREE RADICAL REACTIONS WITH PROTEINS AND ENZYMES - INACTIVATION OF SUBTILISIN CARLSBERG AND SUBTILISIN NOVO [J].
BISBY, RH ;
CUNDALL, RB ;
ADAMS, GE ;
REDPATH, JL .
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1974, 70 :2210-2218
[9]  
CLEMENT GE, 1973, PROG BIOORG CHEM, V2, P177
[10]   IMPLICATION OF PROTEIN AROMATIC AMINO-ACIDS IN CONCANAVALIN A CARBOHYDRATE INTERACTIONS - RADIOLYTIC STUDY [J].
DAVIES, JV ;
MOORE, JS ;
MUDHER, S .
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 1978, 33 (01) :11-19
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