SMALL-ANGLE SCATTERING STUDIES OF PROTEIN-FOLDING

被引：56
作者：
LATTMAN, EE
机构：
[1] Department of Biophysics and Biophysical Chemistry, The Johns Hopkins University School of Medicine, Baltimore
来源：
CURRENT OPINION IN STRUCTURAL BIOLOGY | 1994年 / 4卷 / 01期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.1016/S0959-440X(94)90064-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Small angle scattering experiments can be used to monitor transitions between the folded and denatured states of proteins, or between two different non-native states. These experiments can probe the type of transition, whether two-state or continuous, provide accurate values for the radius of gyration of the states involved, and can signal conformational changes or unusual molecular shapes. The technique is particularly powerful when global changes in size or shape are involved, or when the variety of conformations present precludes the use of crystallography or NMR.
引用
收藏
页码：87 / 92
页数：6
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