EFFECT OF CARBON SOURCE AND DISSOLVED-OXYGEN LEVEL ON CELL-GROWTH AND PULLULANASE PRODUCTION BY BACILLUS-STEAROTHERMOPHILUS G-82

被引：3

作者：

EMANUILOVA, EI ^{[1
]}

KAMBOUROVA, MS ^{[1
]}

机构：

[1] INST MICROBIOL,DEPT ENZYME BIOSYNTHESIS,ACAD G BONCHEV STR B1-26,BU-1113 SOFIA,BULGARIA

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1992年 / 8卷 / 01期

关键词：

PULLULANASE PRODUCTION; BACILLUS-STEAROTHERMOPHILUS; CARBON SOURCE; DISSOLVED OXYGEN;

D O I：

10.1007/BF01200678

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Cell growth and extracellular pullulanase production of Bacillus stearothermophilus G-82 were investigated in batch cultures using a defined medium with glucose, maltose, pullulan or amylopectin as carbon source. Maximum enzyme activity was with pullulan or amylopectin. Cell growth in batch culture was better under oxygen unlimited conditions, while higher total and specific enzyme activities, using pullulan or amylopectin, were obtained in oxygen-limited conditions. Enzyme accumulation took place in the late growth phase. The highest enzyme production of 300 U/l was reached when pullulan was used as carbon source in conditions of oxygen limitation.

引用

页码：21 / 23

页数：3

共 8 条

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