EFFECTS OF RETINOIDS ON MACROPHAGE FUNCTION AND IL-1 ACTIVITY

被引:55
作者
DILLEHAY, DL
WALIA, AS
LAMON, EW
机构
[1] UNIV ALABAMA, DEPT SURG, UNIV STN, BIRMINGHAM, AL 35294 USA
[2] UNIV ALABAMA, BIRMINGHAM VET ADM HOSP, BIRMINGHAM, AL 35294 USA
[3] UNIV ALABAMA, DEPT COMPARAT MED, BIRMINGHAM, AL 35294 USA
[4] UNIV ALABAMA, DEPT MICROBIOL, BIRMINGHAM, AL 35294 USA
关键词
D O I
10.1002/jlb.44.5.353
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The effects of three retinoids, all-trans-retinoic acid (RA), 13-cis-retinoic acid (cRA), and N-(4-hydroxypheny) retinamide (4-HPR), on macrophage function were evaluated. In vitro, RA, cRA, and 4-HPR caused a greater than twofold increase in phagocytosis of IgG-sensitized bovine erthrocytes (IgG-ORBC) by a mouse macrophage cell line (RAW). Significant increases in phagocytosis were produced by retinoid concentrations as low as 2 .times. 10-10 M. RA also significantly increased phagocytosis of IgG-sensitized ORBC by BALB/c peritoneal macrophages in vitro. The ability of RAW macrophages to bind IgG-ORBC was significantly increased by 10-6 to 10-14 M RA. The potentiation of mitogenic responses of spleen cells to Con A and PWM by RA was relatively independent of macrophage function, i.e., splenocytes that were macrophage-depleted were responsive to the potentiating effects of RA. The effects of retinoids on T-cell-dependent B-cell mitogenesis induced by PWM appeared not to be dependent on their previously reported capacity to alter prostaglandin synthesis. Treatment of spleen cells with 10-6 M indomethacin did not abolish the potentiating effects of RA. However, RA in a dose-dependent fashion increased IL-1 activity at the level of the target T-cell. The greatest potentiation of IL-1 activity was at 10-8 M RA. These results show that retinoids can modulate macrophage function at two different levels: potentiation of phagocytosis and potentiation of IL-1 activity at the level of the T-cell.
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页码:353 / 360
页数:8
相关论文
共 37 条
[1]  
BLALOCK JE, 1977, P NATL ACAD SCI USA, V74, P5382, DOI 10.1073/pnas.74.12.5382
[2]  
COHEN BE, 1973, J IMMUNOL, V111, P1376
[3]   EFFECTS OF RETINOIC ACID ON IMMUNE SYSTEM - STIMULATION OF T-KILLER CELL INDUCTION [J].
DENNERT, G ;
LOTAN, R .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1978, 8 (01) :23-29
[4]  
DENNERT G, 1985, CIBA F SYMP, V113, P117
[5]   INVITRO EFFECTS OF RETINOIDS ON MURINE THYMUS-DEPENDENT AND THYMUS-INDEPENDENT MITOGENESIS [J].
DILLEHAY, DL ;
LI, W ;
KALIN, J ;
WALIA, AS ;
LAMON, EW .
CELLULAR IMMUNOLOGY, 1987, 107 (01) :130-137
[6]  
DOVER D, 1982, EXP CELL RES, V138, P193
[7]  
ECCLES SA, 1985, CANCER IMMUNOL IMMUN, V19, P109
[8]  
FORNI G, 1986, JNCI-J NATL CANCER I, V76, P527
[9]  
GOLDFARB RH, 1981, J IMMUNOL, V126, P2129