INVITRO REPLICATION STUDY OF MODIFIED BASES IN RAS SEQUENCES

被引：52
作者：
KAMIYA, H
SAKAGUCHI, T
MURATA, N
FUJIMURO, M
MIURA, H
ISHIKAWA, H
SHIMIZU, M
INOUE, H
NISHIMURA, S
MATSUKAGE, A
MASUTANI, C
HANAOKA, F
OHTSUKA, E
机构：
[1] HOKKAIDO UNIV, FAC PHARMACEUT SCI, KITA-12 NISHI-6, KITA KU, SAPPORO, HOKKAIDO 060, JAPAN
[2] NATL CANC CTR, RES INST, DIV BIOL, CHUO KU, TOKYO 104, JAPAN
[3] AICHI CANC CTR, RES INST, CELL BIOL LAB, CHIKUSA KU, NAGOYA, AICHI 464, JAPAN
[4] RIKEN INST PHYS & CHEM RES, CELLULAR PHYSIOL LAB, WAKO, SAITAMA 35101, JAPAN
来源：
CHEMICAL & PHARMACEUTICAL BULLETIN | 1992年 / 40卷 / 10期
关键词：
O-6-METHYLGUANINE; 8-HYDROXYGUANINE; XANTHINE; HYPOXANTHINE; TAQ DNA POLYMERASE; DNA POLYMERASE-BETA; DNA POLYMERASE-ALPHA; REPLICATION; RAS GENE;
D O I：
10.1248/cpb.40.2792
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
DNA templates containing a modified base (O6-methylguanine, 8-hydroxyguanine, xanthine or hypoxanthine) which was located in nucleotide sequences corresponding to the 12th or 61st codon of a ras gene were synthesized and deoxynucleotide incorporation opposite the lesions was investigated. The templates were replicated by Taq DNA polymerase, recombinant rat DNA polymerase beta and mouse DNA polymerase alpha-primase complex. Sequence analysis of the replicated products indicated selective incorporation of nucleotide(s) opposite a modified base, depending on the kind of base and of DNA polymerase. This system is very useful to obtain results of in vitro replication of modified bases in ras sequences.
引用
收藏
页码：2792 / 2795
页数：4
相关论文
共 27 条
[1]   RAS GENES [J].
BARBACID, M .
ANNUAL REVIEW OF BIOCHEMISTRY, 1987, 56 :779-827
[2]   THE INVIVO MUTAGENIC FREQUENCY AND SPECIFICITY OF O-6-METHYLGUANINE IN PHI-X174 REPLICATIVE FORM DNA [J].
BHANOT, OS ;
RAY, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (19) :7348-7352
[3]   UVRA AND RECA MUTATIONS INHIBIT A SITE-SPECIFIC TRANSITION PRODUCED BY A SINGLE O(6)-METHYLGUANINE IN GENE-G OF BACTERIOPHAGE-PHI-X174 [J].
CHAMBERS, RW ;
SLEDZIEWSKAGOJSKA, E ;
HIRANIHOJATTI, S ;
BOROWYBOROWSKI, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (21) :7173-7177
[4]  
CHENG KC, 1992, J BIOL CHEM, V267, P166
[5]   EXPRESSION OF ACTIVE-RAT DNA POLYMERASE-BETA IN ESCHERICHIA-COLI [J].
DATE, T ;
YAMAGUCHI, M ;
HIROSE, F ;
NISHIMOTO, Y ;
TANIHARA, K ;
MATSUKAGE, A .
BIOCHEMISTRY, 1988, 27 (08) :2983-2990
[6]   SITE-SPECIFIC MUTAGENESIS BY O-6-ALKYLGUANINES LOCATED IN THE CHROMOSOMES OF MAMMALIAN-CELLS - INFLUENCE OF THE MAMMALIAN O-6-ALKYLGUANINE-DNA ALKYLTRANSFERASE [J].
ELLISON, KS ;
DOGLIOTTI, E ;
CONNORS, TD ;
BASU, AK ;
ESSIGMANN, JM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1989, 86 (22) :8620-8624
[7]   SYNTHESIS AND PROPERTIES OF OLIGONUCLEOTIDES CONTAINING 2'-DEOXYNEBULARINE AND 2'-DEOXYXANTHOSINE [J].
ERITJA, R ;
HOROWITZ, DM ;
WALKER, PA ;
ZIEHLERMARTIN, JP ;
BOOSALIS, MS ;
GOODMAN, MF ;
ITAKURA, K ;
KAPLAN, BE .
NUCLEIC ACIDS RESEARCH, 1986, 14 (20) :8135-8153
[8]   PRODUCTION OF SINGLE-STRANDED-DNA TEMPLATES BY EXONUCLEASE DIGESTION FOLLOWING THE POLYMERASE CHAIN-REACTION [J].
HIGUCHI, RG ;
OCHMAN, H .
NUCLEIC ACIDS RESEARCH, 1989, 17 (14) :5865-5865
[9]   SITE-SPECIFIC MUTAGENESIS INVIVO BY SINGLE METHYLATED OR DEAMINATED PURINE-BASES [J].
HILLPERKINS, M ;
JONES, MD ;
KARRAN, P .
MUTATION RESEARCH, 1986, 162 (02) :153-163
[10]   TRANSFORMING ACTIVITY OF A SYNTHETIC C-HA-RAS GENE CONTAINING O6-METHYLGUANINE IN CODON-12 [J].
KAMIYA, H ;
MIURA, K ;
OHTOMO, N ;
NISHIMURA, S ;
OHTSUKA, E .
JAPANESE JOURNAL OF CANCER RESEARCH, 1991, 82 (09) :997-1002
123