COMPARATIVE-ANALYSIS OF HAIR MELANINS BY CHEMICAL AND ELECTRON-SPIN-RESONANCE METHODS

被引：25

作者：

VSEVOLODOV, EB ^{[1
]}

ITO, S ^{[1
]}

WAKAMATSU, K ^{[1
]}

KUCHINA, II ^{[1
]}

LATYPOV, IF ^{[1
]}

机构：

[1] FUJITA HLTH UNIV,SCH HYG,TOYOAKE,AICHI 47011,JAPAN

来源：

PIGMENT CELL RESEARCH | 1991年 / 4卷 / 01期

关键词：

EUMELANIN; PHEOMELANIN; SHEEP; WOOL COLOR; HPLC; ESR;

D O I：

10.1111/j.1600-0749.1991.tb00310.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Eighteen hair samples from Karakul newborn lambs with various colors were estimated for eumelanin and pheomelanin contents (C(e) and C(p), respectively) by electron spin resonance (ESR) spectrometry and by high-performance liquid chromatography (HPLC). Correlation coefficients between the values estimated by the ESR and HPLC methods were 0.96, 0.93, and 0.99 for C(e), C(p), and C(e)/C(p), respectively. The high correlation coefficients show that both methods fit well for estimation of relative values of these parameters. The absolute values of C(e) and C(e)/C(p) coincide rather well when C(e) is high, but considerable discrepancies appear when C(e) is low. The reasons for these discrepancies are discussed. The HPLC method appears to be more sensitive for detection of low concentrations of pheomelanin, while the ESR method fits well for mass selection purposes.

引用

页码：30 / 34

页数：5

共 14 条

[1] MELANOCYTE-STIMULATING HORMONE, TYROSINASE ACTIVITY AND THE REGULATION OF EUMELANOGENESIS AND PHEOMELANOGENESIS IN THE HAIR FOLLICULAR MELANOCYTES OF THE MOUSE [J].