ANALYTICAL AND MICROPREPARATIVE ULTRAHIGH RESOLUTION OF OLIGONUCLEOTIDES BY POLYACRYLAMIDE-GEL HIGH-PERFORMANCE CAPILLARY ELECTROPHORESIS

被引：250
作者：
GUTTMAN, A
COHEN, AS
HEIGER, DN
KARGER, BL
机构：
[1] NORTHEASTERN UNIV,BARNETT INST,BOSTON,MA 02115
[2] NORTHEASTERN UNIV,DEPT CHEM,BOSTON,MA 02115
来源：
ANALYTICAL CHEMISTRY | 1990年 / 62卷 / 02期
关键词：
D O I：
10.1021/ac00201a010
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In continuation of previous work, the separations of poly-deoxyollgonucleotldes on Polyacrylamide gel capillary columns are presented. The use of gel compositions with relatively low monomer content permits columns of very high plate numbers to be obtained. With a 160-mer, plate counts of 30 × 106 per meter are shown. Columns with high precision In relative migration time from run to run, day to day, and batch to batch are presented. Collection of purified fractions from high-resolution electropherograms is also shown to be feasible using field programming techniques. To accomplish this, separation is allowed to occur under high field for resolution and speed, followed by collection under low field where the velocity of the band is purposely decreased in order to widen the time-based width of the band. © 1990, American Chemical Society. All rights reserved.
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页码：137 / 141
页数：5
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