FLUOROMETRIC SCANNING OF POLYACRYLAMIDE GELS

被引：6
作者：
BORRIS, DP
ARONSON, JN
机构：
[1] Department of Chemistry, State University of New York at Albany, Albany
来源：
ANALYTICAL BIOCHEMISTRY | 1969年 / 32卷 / 02期
基金：
美国国家科学基金会;
关键词：
D O I：
10.1016/0003-2697(69)90085-2
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A gel scanning technique that measures the quench of N-allyl-DANSA fluorescence by amido schwarz stained protein bands had the potential capability of about fifty times the sensitivity of absorption densitometric techniques. The preparation of the fluorescent polyacrylamide gels was straightforward and their electrophoretic resolving and storage properties compared favorably with standard gels. An inexpensive modification of a common commercial fluorometer was described for scanning these gels. © 1969.
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页码：273 / &
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