IDENTIFICATION OF UNNATURAL PHASEIC ACID AS A METABOLITE DERIVED FROM EXOGENOUSLY ADDED (-)-ABSCISIC ACID IN A MAIZE CELL-SUSPENSION CULTURE

被引：15

作者：

BALSEVICH, JJ ^{[1
]}

ABRAMS, SR ^{[1
]}

LAMB, N ^{[1
]}

KONIG, WA ^{[1
]}

机构：

[1] UNIV HAMBURG,INST ORGAN CHEM,W-2000 HAMBURG 13,GERMANY

来源：

PHYTOCHEMISTRY | 1994年 / 36卷 / 03期

关键词：

ZEA MAYS; GRAMINEAE; CELL CULTURE; METABOLISM; ABSCISIC ACID; PHASEIC ACID; CHIRAL GAS CHROMATOGRAPHY;

D O I：

10.1016/S0031-9422(00)89790-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Unnatural phaseic acid (U-PA) was identified by chiral GC as a minor component of the metabolite mixture obtained from the medium of a maize cell suspension culture treated with unnatural (-)-(R)-abscisic acid (U-ABA). When U-[3',5',5',7',7',7']-hexadeutero-ABA was fed to the cell suspension, d(6)-PA was obtained, establishing its origin from the exogenously-added U-ABA. The major metabolite produced was identified as U-7'-hydroxyabscisic acid (U-7'-HOABA) [d(5)-7'-HOABA from d(6)-U-ABA]. Confirmation of the assignments was obtained by HPLC/LC/CFSIMS analysis of the metabolite mixtures. This result corroborates a previous report of the formation of U-PA from racemic ABA in avocado mesocarp tissue.

引用

页码：647 / 650

页数：4

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