CLONING AND CHROMOSOMAL ORGANIZATION OF A GENE ENCODING A PUTATIVE AMINO-ACID PERMEASE FROM SACCHAROMYCES-CEREVISIAE

被引：11

作者：

MAI, B ^{[1
]}

LIPP, M ^{[1
]}

机构：

[1] LUDWIG MAXIMILIANS UNIV MUNCHEN,INST BIOCHEM,D-82152 MARTINSRIED,GERMANY

来源：

GENE | 1994年 / 143卷 / 01期

关键词：

RECOMBINANT DNA; AMINO-ACID TRANSPORT; MEMBRANE PROTEIN; CHROMOSOME IV; TRANSCRIPT MAPPING; FUNGAL GENE;

D O I：

10.1016/0378-1119(94)90617-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A new member of the yeast amino acid (aa) permease gene family has been cloned, mapped and sequenced. The sequence of the PAP1 (putative aa permease 1) gene contains an open reading frame of 566 aa corresponding to a polypeptide with a calculated M(r) of 62704. Its hydropathy profile suggests the presence of 13 membrane-spanning regions and a charged N-terminal domain. It does not resemble hydrophobic signal sequences found in secreted proteins. Hence, PAP1 encodes a protein with characteristics typical of integral membrane proteins translocating ligands across cellular membranes. Sequence comparisons indicate strong homology to the five known aa permeases of Saccharomyces cerevisiae and to an aa transporter in Trichoderma harzianum. Primer extension analysis revealed one major and one minor transcription start point located 121 and 125 nucleotides upstream from the ATG start codon, corresponding to a 2.1-kb transcript. PAP1 was mapped in a contig of three known (DBF4, TPI and HEM12), but so far unlinked, genes on chromosome IV.

引用

页码：129 / 133

页数：5

共 18 条

[1]

Ausubel FM., 1995, MOL REPROD DEV, V3rd edn, DOI DOI 10.1002/MRD.1080010210

[2]

BENNETZEN JL, 1982, J BIOL CHEM, V257, P3026

[3] MOLECULAR MECHANISMS OF PROTEIN SECRETION - THE ROLE OF THE SIGNAL SEQUENCE [J].

BRIGGS, MS ;

GIERASCH, LM .

ADVANCES IN PROTEIN CHEMISTRY, 1986, 38 :109-180

[4]

Dobner T, 1991, DNA Seq, V1, P409, DOI 10.3109/10425179109020799

[5]

DUNNEN JT, 1993, HUMAN GENETIC DISEAS, P35

[6] ORGANIZATION AND SEQUENCES AT THE 5' END OF A CLONED COMPLETE OVALBUMIN GENE [J].

GANNON, F ;

OHARE, K ;

PERRIN, F ;

LEPENNEC, JP ;

BENOIST, C ;

COCHET, M ;

BREATHNACH, R ;

ROYAL, A ;

GARAPIN, A ;

CAMI, B ;

CHAMBON, P .

NATURE, 1979, 278 (5703) :428-434

[7] POSITIVE REGULATION IN THE GENERAL AMINO-ACID CONTROL OF SACCHAROMYCES-CEREVISIAE [J].

HINNEBUSCH, AG ;

FINK, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (17) :5374-5378

[8]

HOFFMANN W, 1985, J BIOL CHEM, V260, P1831

[9] GAP1, THE GENERAL AMINO-ACID PERMEASE GENE OF SACCHAROMYCES-CEREVISIAE - NUCLEOTIDE-SEQUENCE, PROTEIN SIMILARITY WITH THE OTHER BAKERS-YEAST AMINO-ACID PERMEASES, AND NITROGEN CATABOLITE REPRESSION [J].

JAUNIAUX, JC ;

GRENSON, M .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1990, 190 (01) :39-44

[10] ASSEMBLY OF ASPARAGINE-LINKED OLIGOSACCHARIDES [J].

KORNFELD, R ;

KORNFELD, S .

ANNUAL REVIEW OF BIOCHEMISTRY, 1985, 54 :631-664

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