EVIDENCE THAT THE ACTIVE-CENTER OF CHYMOPAPAIN-A IS DIFFERENT FROM THE ACTIVE-CENTERS OF SOME OTHER CYSTEINE PROTEINASES AND THAT THE BRONSTED COEFFICIENT (BETA-NUC.) FOR THE REACTIONS OF THIOLATE ANIONS WITH 2,2'-DIPYRIDYL DISULFIDE MAY BE DECREASED BY REAGENT PROTONATION

被引：11

作者：

BROCKLEHURST, K

BAINES, BS

MUSHIRI, MS

机构：

来源：

BIOCHEMICAL JOURNAL | 1980年 / 189卷 / 01期

关键词：

D O I：

10.1042/bj1890189

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：189 / 192

页数：4

共 17 条

[1]

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[2] NECESSARY MODIFICATION TO THE PREPARATION OF PAPAIN FROM ANY HIGH-QUALITY LATEX OF CARICA-PAPAYA AND EVIDENCE FOR THE STRUCTURAL INTEGRITY OF THE ENZYME PRODUCED BY TRADITIONAL METHODS [J].

BAINES, BS ;

BROCKLEHURST, K .

BIOCHEMICAL JOURNAL, 1979, 177 (02) :541-548

[3] REACTIVITIES OF NEUTRAL AND CATIONIC FORMS OF 2,2'-DIPYRIDYL DISULFIDE TOWARDS THIOLATE ANIONS - DETECTION OF DIFFERENCES BETWEEN THE ACTIVE-CENTERS OF ACTINIDIN, PAPAIN AND FICIN BY A 3-PROTONIC-STATE REACTIVITY PROBE [J].

BROCKLEHURST, K ;

STUCHBURY, T ;

MALTHOUSE, JPG .

BIOCHEMICAL JOURNAL, 1979, 183 (02) :233-238

[4] EVIDENCE THAT BINDING TO THE S-2-SUBSITE OF PAPAIN MAY BE COUPLED WITH CATALYTICALLY RELEVANT STRUCTURAL-CHANGE INVOLVING THE CYSTEINE-25-HISTIDINE-159 DIAD - KINETICS OF THE REACTION OF PAPAIN WITH A 2-PROTONIC-STATE REACTIVITY PROBE CONTAINING A HYDROPHOBIC SIDE-CHAIN [J].

BROCKLEHURST, K ;

MALTHOUSE, JPG ;

SHIPTON, M .

BIOCHEMICAL JOURNAL, 1979, 183 (02) :223-231

[5] REACTIONS OF PAPAIN AND OF LOW-MOLECULAR-WEIGHT THIOLS WITH SOME AROMATIC DISULFIDES - 2,2'-DIPYRIDYL DISULFIDE AS A CONVENIENT ACTIVE-SITE TITRANT FOR PAPAIN EVEN IN PRESENCE OF OTHER THIOLS [J].

BROCKLEHURST, K ;

LITTLE, G .

BIOCHEMICAL JOURNAL, 1973, 133 (01) :67-80

[6]

CAYLE THEODORE, 1964, WALLERSTEIN LAB COMMUN, V27, P87

[7]

EBATA M, 1962, J BIOL CHEM, V237, P1086

[8] REACTIONS OF NUCLEOPHILIC REAGENTS WITH ACYLATING AGENTS OF EXTREME REACTIVITY AND UNREACTIVITY - CORRELATION OF BETA VALUES FOR ATTACKING AND LEAVING GROUP VARIATION [J].

FERSHT, AR ;

JENCKS, WP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1970, 92 (18) :5442-&

[9]

FROST AA, 1961, KINETICS MECHANISM, P160

[10] NONLINEAR STRUCTURE-REACTIVITY CORRELATIONS - ACYL TRANSFER BETWEEN SULFUR AND OXYGEN NUCLEOPHILES [J].

HUPE, DJ ;

JENCKS, WP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1977, 99 (02) :451-464

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