INVOLVEMENT OF ECTO-PHOSPHORYL TRANSFER IN CONTRACTIONS OF THE SMOOTH-MUSCLE OF THE GUINEA-PIG VAS-DEFERENS TO ADENOSINE 5'-TRIPHOSPHATE

Extracellular ATP (> 3 x 10(-5) M) elicits a transient, biphasic contraction of the vas deferens. The specific P2x-purinoceptor photoaffinity label antagonist, arylazido aminopropionyl ATP (ANAPP3), inhibits the first contractile phase. The second phase, which is increased for adenosine 5'-O-(3-thio-triphosphate)(ATP-gamma-S) and attenuated for 5'-anhydride-substituted ATP analogs, has been hypothesized to be initiated by phosphate chain cleavage, and is inhibited irreversibly by periodate-oxidized ATP (P-ATP), an affinity label. We examined whether phosphorylation occurs during contraction to ATP-gamma-S and ATP, and whether the irreversible inhibition of the second phase of contraction by P-ATP results from its covalent incorportation. After incubation of intact tissues with [S-35]ATP-gamma-S (3 mM), homogenization, sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography, (SPO2(3-))-S-35 was incorporated predominantly into a single, 19 to 21 kD protein. The incorporation was transient and paralleled the time course of the transient contraction to ATP-gamma-S (peak by 5 to 10 sec, then rapid decline during 60 sec). Treatment with P-ATP (10 mM; 5 min) or ANAPP3 (10(-4) M) inhibited S-35 incorporation at 60 sec. (PO4(3-))-P-32 from [gamma-P-32] ATP (3 mM) also was incorporated rapidly into the protein; removal of P-32, however, was faster than removal of S-35. [H-3]P-ATP was incorporated into several proteins, but not into the 19 to 21 kD protein nor the ones labeled by photolyzed ANAPP3. Incorporation of S-35 from [S-35]ATP-gamma-S, and [H-3]P-ATP, were not affected by incubation in the presence of histamine or norepinephrine (10(-4)-10(-3) M), whereas ATP (10(-2) M) reduced incorporation of [H-3]P-ATP (10(-5)-10(-2) M). The results support the hypothesis that the second phase of contraction to ATP involves a transductive phosphoryl transfer involving an ecto-kinase, which is inhibited by P-ATP.