GLUCOSEOXIDASE FROM ASPERGILLUS-NIGER IN REVERSE MICELLES - PH AND W(O) DEPENDENCE

The well known enzymatic method for the determination of glucose in aqueous solution with the system glucose oxidase/peroxidase/o-dianisidine was modified in a way that enables the quantitative determination of glucose in various reverse micellar systems. For instance in the 0.1 M sodium-bis-(2-ethylhexyl)-sulfosuccinate/n-octane systeme the useable glucose measuring range is between 10 and 70 mu g glucose per 3 ml micellar solution. Investigations concerning the influence of the pH-value and the w(o)-value on the enzyme activity of glucose oxidase in the syst em 0.1 M sodium-bis-(2-ethylhexyl)sulfosuccinate/n-octane led to a shift of the pH-optimum from pH 5.5-6.0 in aqueous solution, to pH 4.0, in reverse micellar solution. The bell-shaped w(o)-dependence of the enzyme activity, typical for many other enzymes, was not found for glucose oxidase at pH 4.0, and was less clearly visible at the other investigated pH-values with an optimum at w(o)=5.6.