MOLECULAR GENETIC-ANALYSIS OF THE ABO BLOOD-GROUP SYSTEM .1. WEAK SUBGROUPS - A3 AND B3 ALLELES

被引：101

作者：

YAMAMOTO, FI ^{[1
]}

MCNEILL, PD ^{[1
]}

YAMAMOTO, M ^{[1
]}

HAKOMORI, SI ^{[1
]}

HARRIS, T ^{[1
]}

JUDD, WJ ^{[1
]}

DAVENPORT, RD ^{[1
]}

机构：

[1] UNIV WASHINGTON,DEPT PATHOBIOL,SEATTLE,WA 98195

来源：

VOX SANGUINIS | 1993年 / 64卷 / 02期

关键词：

D O I：

10.1111/j.1423-0410.1993.tb02528.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We have determined the nucleotide sequences of the coding region in the last two coding exons of ABO genes (which occupy 91% of the soluble form of A1 transferase) from 7 individuals with weak subgroup phenotypes. Four of the individuals had an A3 phenotype and 3 individuals had a B3 phenotype. We determined the nucleotide sequences based on PCR followed by subcloning and DNA sequencing of the amplified fragments. Two cases of the A3 allele and 1 case of the B3 allele were found to contain a single-base substitution which resulted in an amino acid substitution. However, no other cases of A3 and B3 alleles were found to contain differences in this region. This finding demonstrates for the first time heterogeneity among these weak subgroups at the nucleotide level.

引用

页码：116 / 119

页数：4

共 13 条

[1]

CARTRON JP, 1978, J IMMUNOGENET, V5, P107

[2]

ISSIT P, 1985, APPLIED BLOOD GROUP

[3]

Landsteiner K., 1900, ZENTRALBL BAKTERIOL, V27, P357

[4]

Maniatis T., 1982, MOL CLONING

[5]

NAKAMURA I, 1989, EXP CLIN IMMUNOGENET, V6, P143

[6]

Race RR, 1975, BLOOD GROUPS MAN

[7] ENZYMATIC AMPLIFICATION OF BETA-GLOBIN GENOMIC SEQUENCES AND RESTRICTION SITE ANALYSIS FOR DIAGNOSIS OF SICKLE-CELL ANEMIA [J].

SAIKI, RK ;

SCHARF, S ;

FALOONA, F ;

MULLIS, KB ;

HORN, GT ;

ERLICH, HA ;

ARNHEIM, N .

SCIENCE, 1985, 230 (4732) :1350-1354

[8]

Salmon C, 1984, HUMAN BLOOD GROUPS

[9] DNA SEQUENCING WITH CHAIN-TERMINATING INHIBITORS [J].

SANGER, F ;

NICKLEN, S ;

COULSON, AR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5463-5467

[10]

YAMAMOTO F, 1990, J BIOL CHEM, V265, P19257

← 1 2 →