HPLC SEPARATION OF COPPER(II), NICKEL(II) AND VANADIUM(IV) OXIDE COMPLEXES OF TETRADENTATE LIGANDS DERIVED FROM SALICYLALDEHYDE AND SUBSTITUTED ETHYLENEDIAMINES

The copper(II), nickel(II) and vanadium (IV) oxide complexes of a new tetradentate ligand bis (salicylaldehyde)-ethylmethylethylenediimine (H2SA2EM) have been prepared; and characterized using, IR, mass, H-1-NMR, UV and visible spectrophtometry. The metal complexes together with the copper, nickel and vanadium oxide complexes of meso-and dl-bis(salicylaldehyde)-stilbenediimine are separated on reversed phase HPLC on the column Zorbax ODS (250 x 4.6 mm) with guard column ODS. The complexes were eluted isocretically with two to four component solvent system. Detection was achieved using UV detector. Optimum conditions for the separation of the metal complexes, calibration ranges and detection limits are reported. The interfering effect of the excess of the reagent on the separation and the effect of the substituents at ethylene bridge are also reported.