EFFLUX OF BIS-CARBOXYETHYL-CARBOXYFLUORESCEIN (BCECF) BY A NOVEL ATP-DEPENDENT TRANSPORT MECHANISM IN EPITHELIAL-CELLS

被引：28

作者：

ALLEN, CN ^{[1
]}

HARPUR, ES ^{[1
]}

GRAY, TJB ^{[1
]}

SIMMONS, NL ^{[1
]}

HIRST, BH ^{[1
]}

机构：

[1] STERLING WINTHROP RES CTR,ALNWICK NE66,NORTHD,ENGLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)80203-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The efflux of the intracellular pH fluorochrome 2′, 7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) was quantified in four cultured epithelial cell lines; HCT-8, T84, HGT-1 and MDCK. BCECF efflux was time-dependent, and after 5 h 45-91% of the initial BCECF loaded was extracellular, efflux being greatest in MDCK cells. Depletion of cellular ATP approximately halved BCECF efflux. BCECF efflux was inhibited by indomethacin, vinblastine and verapamil, but not by nifedipine or reserpine. Certain features of BCECF efflux resemble drug efflux in multidrug resistant cells, but inhibition of efflux displays a distinct pharmacological profile suggesting BCECF is a substrate for a novel ATP-dependent transport system. © 1990 Academic Press, Inc.

引用

页码：262 / 267

页数：6

共 21 条

[1] THE CELL BIOLOGY OF MULTIPLE-DRUG RESISTANCE [J].