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SEQUENCE OF THE RABBIT GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE-ENCODING CDNA
被引:41
|作者:
APPLEQUIST, SE
KEYNA, U
CALVIN, MR
BECKENGESER, GB
RAMAN, C
JACK, HM
机构:
[1] LOYOLA UNIV,STRITCH SCH MED,DEPT MICROBIOL & IMMUNOL,MAYWOOD,IL 60153
[2] LOYOLA UNIV,STRITCH SCH MED,PROGRAM MOLEC BIOL,MAYWOOD,IL 60153
[3] ROSARY COLL,DEPT BIOL,RIVER FOREST,IL 60305
来源:
关键词:
CDNA CLONING;
POLYMERASE CHAIN REACTION;
IN VITRO TRANSLATION;
SEQUENCE HOMOLOGY;
D O I:
10.1016/0378-1119(95)00386-K
中图分类号:
Q3 [遗传学];
学科分类号:
071007 ;
090102 ;
摘要:
Two full-length cDNA clones encoding rabbit glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were isolated from a lambda gt10 rabbit spleen cDNA library and sequenced. As predicted from the open reading frame (ORF) in vitro translation of a sense orientation GAPDH cDNA clone yielded a protein product with a molecular mass of 37 kDa. Rabbit GAPDH exhibits a high degree of homology to the mouse, rat, hamster, chicken and human GAPDH on both the nucleotide (nt) and amino acid (aa) levels.
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页码:325 / 326
页数:2
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