SEQUENCE OF THE RABBIT GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE-ENCODING CDNA

被引：41

作者：

APPLEQUIST, SE

KEYNA, U

CALVIN, MR

BECKENGESER, GB

RAMAN, C

JACK, HM

机构：

[1] LOYOLA UNIV,STRITCH SCH MED,DEPT MICROBIOL & IMMUNOL,MAYWOOD,IL 60153

[2] LOYOLA UNIV,STRITCH SCH MED,PROGRAM MOLEC BIOL,MAYWOOD,IL 60153

[3] ROSARY COLL,DEPT BIOL,RIVER FOREST,IL 60305

来源：

GENE | 1995年 / 163卷 / 02期

关键词：

CDNA CLONING; POLYMERASE CHAIN REACTION; IN VITRO TRANSLATION; SEQUENCE HOMOLOGY;

D O I：

10.1016/0378-1119(95)00386-K

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two full-length cDNA clones encoding rabbit glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were isolated from a lambda gt10 rabbit spleen cDNA library and sequenced. As predicted from the open reading frame (ORF) in vitro translation of a sense orientation GAPDH cDNA clone yielded a protein product with a molecular mass of 37 kDa. Rabbit GAPDH exhibits a high degree of homology to the mouse, rat, hamster, chicken and human GAPDH on both the nucleotide (nt) and amino acid (aa) levels.

引用

页码：325 / 326

页数：2

共 6 条

[1] VARIOUS RAT ADULT TISSUES EXPRESS ONLY ONE MAJOR MESSENGER-RNA SPECIES FROM THE GLYCERALDEHYDE-3-PHOSPHATE-DEHYDROGENASE MULTIGENIC FAMILY [J].