SUBSETS OF CD34(+) CELLS AND RAPID HEMATOPOIETIC RECOVERY AFTER PERIPHERAL-BLOOD STEM-CELL TRANSPLANTATION

被引:127
作者
DERCKSEN, MW
RODENHUIS, S
DIRKSON, MKA
SCHAASBERG, WP
BAARS, JW
VANDERWALL, E
SLAPERCORTENBACH, ICM
PINEDO, HM
VONDEMBORNE, AEGK
VANDERSCHOOT, CE
GERRITSEN, WR
机构
[1] NETHERLANDS RED CROSS, BLOOD TRANSFUS SERV, CENT LAB, DEPT EXPTL IMMUNOHEMATOL, 1006 AD AMSTERDAM, NETHERLANDS
[2] EUROPEAN CANC CTR, AMSTERDAM, NETHERLANDS
[3] FREE UNIV AMSTERDAM HOSP, DEPT MED ONCOL, 1081 HV AMSTERDAM, NETHERLANDS
[4] ANTONI VAN LEEUWENHOEK HOSP, NETHERLANDS CANC INST, DEPT MED ONCOL IMMUNOL, AMSTERDAM, NETHERLANDS
[5] UNIV AMSTERDAM, ACAD MED CTR, DEPT HEMATOL, 1105 AZ AMSTERDAM, NETHERLANDS
关键词
D O I
10.1200/JCO.1995.13.8.1922
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Purpose: To study whether there is a relationship between transplanted cell dose and rate of hematopoietic recovery after peripheral-blood stem-cell (PBSC) transplantation, and to obtain an indication whether specific subsets of CD34(+) cell populations contribute to rapid recovery of neutrophils or platelets. Patients and Methods: based on data from 59 patients, we calculated for each day after PBSC transplantation the dose of CD34(+) cells that resulted in rapid recovery of either neutrophils or platelets in the majority (> 70%) of patients. Using dual-color how cytometry, subsets of peripheral-blood CD34(+) cells were quantified and the numbers of CD34(+) cells belonging to each of the reinfused subsets correlated with hematopoietic recovery following high-dose chemotherapy. Results: The calculated threshold values with a high probability of engraftment showed a steep dose-effect relationship between CD34(+) cell dose and time to recovery of both neutrophils or platelets. predominantly CD34(+) cells with the phenotype of myeloid precursors were mobilized. A minority of CD34(+) cells expressed the erythroid and megakaryocytic lineage-associated antigens and ct low but distinct population of CD34(+) cells expressed antigens associated with multipotent stem cells. Analysis showed that the number of CD34(+)CD33(-) cells (r = -.74, P < .05), as well as the number of CD34(+)CD41(+) cells (r = -.81, P < .005), correlated significantly better with time to neutrophil and platelet recovery, respectively, than with the total number of CD34(+) cells (r = -.55 and r = -.56, respectively). Conclusion: The numbers of CD34(+)CD33(-) cells and CD34(+)CD41(+) cells may help to predict short-term repopulation capacity of pBSCs, especially when relatively low numbers of CD34(+) cells per kilogram are reinfused. J Clin Oncol 13:1922-1932. (C) 1995 by American Society of Clinical Oncology.
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页码:1922 / 1932
页数:11
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