A HIGHLY SENSITIVE METHOD FOR DETECTION OF MOLYBDENUM-CONTAINING PROTEINS

被引：0
|
作者：
KALAKUTSKII, KL [1 ]
SHVETSOV, AA [1 ]
BURSAKOV, SA [1 ]
LETAROV, AV [1 ]
ZABOLOTNYI, AI [1 ]
LVOV, NP [1 ]
机构：
[1] BF KUPREVICH EXPTL BOT INST, MINSK, BELARUS
来源：
BIOCHEMISTRY-MOSCOW | 1992年 / 57卷 / 06期
关键词：
MOLYBDENUM; TUNGSTEN; MOLYBDENUM-CONTAINING PROTEINS;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A highly sensitive method for detection of molybdenum-containing proteins in gels after electrophoresis has been developed. The method involves in vitro labeling of the proteins with the radioactive isotope W-185. The method was used to detect molybdenum-accumulating proteins in lupine seeds, xanthine dehydrogenase and another molybdenum-containing protein in wheat, barley, and pea seedlings, and nitrate reductase and xanthine dehydrogenase in bacteroides from lupine nodules. Nitrogenase could not be detected by the method.
引用
收藏
页码：624 / 629
页数：6
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