A HIGHLY SENSITIVE METHOD FOR DETECTION OF MOLYBDENUM-CONTAINING PROTEINS

被引：0

作者：

KALAKUTSKII, KL ^{[1
]}

SHVETSOV, AA ^{[1
]}

BURSAKOV, SA ^{[1
]}

LETAROV, AV ^{[1
]}

ZABOLOTNYI, AI ^{[1
]}

LVOV, NP ^{[1
]}

机构：

[1] BF KUPREVICH EXPTL BOT INST, MINSK, BELARUS

来源：

BIOCHEMISTRY-MOSCOW | 1992年 / 57卷 / 06期

关键词：

MOLYBDENUM; TUNGSTEN; MOLYBDENUM-CONTAINING PROTEINS;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A highly sensitive method for detection of molybdenum-containing proteins in gels after electrophoresis has been developed. The method involves in vitro labeling of the proteins with the radioactive isotope W-185. The method was used to detect molybdenum-accumulating proteins in lupine seeds, xanthine dehydrogenase and another molybdenum-containing protein in wheat, barley, and pea seedlings, and nitrate reductase and xanthine dehydrogenase in bacteroides from lupine nodules. Nitrogenase could not be detected by the method.

引用

页码：624 / 629

页数：6

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