A COMPARATIVE-STUDY OF EFFICIENCIES OF FIBER OPTIC AND PRISM TIRF SENSORS

被引：4

作者：

CELEBRE, M

DOMENICI, C

FRANCESCONI, R

AHLUWALIA, A

SCHIRONE, A

机构：

[1] ENEA, CRAM, LA SPEZIA, ITALY

[2] CNR, INST CLIN PHYSIOL, I-56100 PISA, ITALY

来源：

MEASUREMENT SCIENCE AND TECHNOLOGY | 1992年 / 3卷 / 12期

关键词：

D O I：

10.1088/0957-0233/3/12/009

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A comparative study of the respective performances of total internal reflection fluorescence (TIRF) prisms and fibre optic sensors in terms of their detection capabilities is presented. Theoretical models based on geometrical considerations describe the excitation and collection efficiencies of the two optical systems. The models are shown to be in excellent agreement with the experimental TIRF data obtained from the adsorption of albumin onto prism or fibre surfaces. Improvements in system performance, based on optimization of the optical configurations, have been evaluated. The studies demonstrate that, in terms of the requirements of biosensor devices, in which compactness and high efficiencies are paramount, fibre optic systems are superior to prism systems.

引用

页码：1166 / 1173

页数：8

共 16 条

[1] A COMPARATIVE-STUDY OF PROTEIN IMMOBILIZATION TECHNIQUES FOR OPTICAL IMMUNOSENSORS [J].