CLONING AND EXPRESSION OF A CHITINASE GENE FROM AEROMONAS-HYDROPHILA IN ESCHERICHIA-COLI

被引：29
作者：
CHEN, JP
NAGAYAMA, F
CHANG, MC
机构：
[1] DEPT FOOD & AGR, COUNCIL AGR, TAIPEI, TAIWAN
[2] TOKYO UNIV FISHERIES, DEPT FOOD SCI & TECHNOL, TOKYO 108, JAPAN
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1991年 / 57卷 / 08期
关键词：
D O I：
10.1128/AEM.57.8.2426-2428.1991
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An extracellular secreted chitinase gene from Aeromonas hydrophila was cloned in Escherichia coli, and the gene product was detected in the culture medium. Like the natural chitinase protein, the excreted chitinase had a molecular weight of approximately 85,000 and was subject to catabolite repression by glucose.
引用
收藏
页码：2426 / 2428
页数：3
相关论文
共 27 条
[1]  
[Anonymous], 2013, CHITIN
[2]   TRANSFORMATION OF YEAST BY A REPLICATING HYBRID PLASMID [J].
BEGGS, JD .
NATURE, 1978, 275 (5676) :104-109
[3]   ETHYLENE-REGULATED GENE-EXPRESSION - MOLECULAR-CLONING OF THE GENES ENCODING AN ENDOCHITINASE FROM PHASEOLUS-VULGARIS [J].
BROGLIE, KE ;
GAYNOR, JJ ;
BROGLIE, RM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (18) :6820-6824
[4]   CLONING, EXPRESSION, AND MAPPING OF THE AEROMONAS-HYDROPHILA AEROLYSIN GENE DETERMINANT IN ESCHERICHIA-COLI K-12 [J].
CHAKRABORTY, T ;
HUHLE, B ;
BERGBAUER, H ;
GOEBEL, W .
JOURNAL OF BACTERIOLOGY, 1986, 167 (01) :368-374
[5]   CLONING OF ENTERO-TOXIN GENE FROM AEROMONAS-HYDROPHILA PROVIDES CONCLUSIVE EVIDENCE OF PRODUCTION OF A CYTOTONIC ENTERO-TOXIN [J].
CHAKRABORTY, T ;
MONTENEGRO, MA ;
SANYAL, SC ;
HELMUTH, R ;
BULLING, E ;
TIMMIS, KN .
INFECTION AND IMMUNITY, 1984, 46 (02) :435-441
[6]   CLONING OF A SERRATIA-MARCESCENS GENE ENCODING CHITINASE [J].
FUCHS, RL ;
MCPHERSON, SA ;
DRAHOS, DJ .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1986, 51 (03) :504-509
[7]   MOLECULAR-CLONING, CHARACTERIZATION, AND NUCLEOTIDE-SEQUENCE OF AN EXTRACELLULAR AMYLASE GENE FROM AEROMONAS-HYDROPHILA [J].
GOBIUS, KS ;
PEMBERTON, JM .
JOURNAL OF BACTERIOLOGY, 1988, 170 (03) :1325-1332
[8]   MOLECULAR-CLONING AND EXPRESSION IN ESCHERICHIA-COLI OF THE STRUCTURAL GENE FOR THE HEMOLYTIC TOXIN AEROLYSIN FROM AEROMONAS-HYDROPHILA [J].
HOWARD, SP ;
BUCKLEY, JT .
MOLECULAR & GENERAL GENETICS, 1986, 204 (02) :289-295
[9]   BIOCHEMICAL AND EXOENZYMATIC PROPERTIES OF AEROMONAS SPECIES [J].
JANDA, JM .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1985, 3 (03) :223-232
[10]   EXPRESSION OF BACTERIAL CHITINASE PROTEIN IN TOBACCO-LEAVES USING 2 PHOTOSYNTHETIC GENE PROMOTERS [J].
JONES, JDG ;
DEAN, C ;
GIDONI, D ;
GILBERT, D ;
BONDNUTTER, D ;
LEE, R ;
BEDBROOK, J ;
DUNSMUIR, P .
MOLECULAR & GENERAL GENETICS, 1988, 212 (03) :536-542
123