HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SEPARATION OF HUMAN APOLACTOFERRIN AND MONOFERRIC AND DIFERRIC LACTOFERRINS

被引：26
作者：
MAKINO, Y
NISHIMURA, S
机构：
[1] Department of Biochemistry, School of Medicine, Showa University, Shinagawa-ku, Tokyo, 142
来源：
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1992年 / 579卷 / 02期
关键词：
D O I：
10.1016/0378-4347(92)80402-C
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A simple chromatographic separation of the different molecular forms of human milk lactoferrin according to their iron contents is described. The appropriate conditions were developed with a Mono-S cation-exchange column finked to an automated Urtrochrom GTi system. The pure lactoferrin with different iron binding (apolactoferrin, monoferric lactoferrin and diferric lactoferrin) separated into three peaks. The three peaks were eluted from the Mono-S column with 0.88, 0.97 and 1.05 M sodium chloride in 10 mM phosphate buffer (pH 7.0).
引用
收藏
页码：346 / 349
页数：4
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