SEMIPERMEABLE-SURFACE REVERSED-PHASE MEDIA FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引:128
作者
DESILETS, CP [1 ]
ROUNDS, MA [1 ]
REGNIER, FE [1 ]
机构
[1] PURDUE UNIV,DEPT CHEM,W LAFAYETTE,IN 47907
来源
JOURNAL OF CHROMATOGRAPHY | 1991年 / 544卷 / 1-2期
关键词
D O I
10.1016/S0021-9673(01)83976-5
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Polyoxyethylene was both adsorbed hydrophobically (through the use of non-ionic surfactants) and covalently bonded to reversed-phase high-performance liquid chromatographic packings, thereby establishing a semipermeable hydrophilic layer over the alkylsilane surface. This layer restricts proteins from adsorbing to the alkylsilane phase while permitting penetration and chromatographic separation of small molecules. Biological fluids containing low-molecular-weight analytes may be injected directly, without sample pretreatment or the use of micellar eluents. In the case of adsorbed coatings, surfactant loading was determined primarily by the surface area (over the reversed phase) occupied by the polyoxyethylene head group. Semipermeability of the hydrophilic layer was demonstrated by observing changes in retention of both small molecules and proteins with increasing eluent ionic strength. Coated column stability was evaluated with regard to cumulative eluent volume and repeated serum injections.
引用
收藏
页码:25 / 39
页数:15
相关论文
共 30 条
[1]   MICELLES IN SEPARATIONS - A PRACTICAL AND THEORETICAL REVIEW [J].
ARMSTRONG, DW .
SEPARATION AND PURIFICATION METHODS, 1985, 14 (02) :213-304
[2]   CHARACTERIZATION AND EVALUATION OF THE USE OF NONIONIC POLYOXYETHYLENE(23)DODECANOL MICELLAR MOBILE PHASES IN REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
BORGERDING, MF ;
HINZE, WL .
ANALYTICAL CHEMISTRY, 1985, 57 (12) :2183-2190
[3]   INVESTIGATIONS OF STATIONARY PHASE MODIFICATION BY THE MOBILE PHASE SURFACTANT IN MICELLAR LIQUID-CHROMATOGRAPHY [J].
BORGERDING, MF ;
HINZE, WL ;
STAFFORD, LD ;
FULP, GW ;
HAMLIN, WC .
ANALYTICAL CHEMISTRY, 1989, 61 (13) :1353-1358
[4]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[5]   EFFECT OF SURFACTANTS ON THE SEPARATION OF PROTEINS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY .1. NON-IONIC SURFACTANTS (TWEEN) [J].
CHANG, JP .
JOURNAL OF CHROMATOGRAPHY, 1984, 317 (DEC) :157-163
[6]   SILICA-BOUND POLYETHYLENEGLYCOL AS STATIONARY PHASE FOR SEPARATION OF PROTEINS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY [J].
CHANG, JP ;
ELRASSI, Z ;
HORVATH, C .
JOURNAL OF CHROMATOGRAPHY, 1985, 319 (03) :396-399
[7]  
CLUNIE JS, 1983, ADSORPTION SOLUTION, pCH3
[8]  
DESILETS CP, 1990, Patent No. 7488681
[9]  
DESILETS CP, 1988, THESIS PURDUE U W LA
[10]  
DORSEY JG, 1987, ADV CHROMATOGR, V27, P168