YEAST COATOMER CONTAINS A SUBUNIT HOMOLOGOUS TO MAMMALIAN BETA'-COP

被引：20

作者：

HARTER, C ^{[1
]}

DRAKEN, E ^{[1
]}

LOTTSPEICH, F ^{[1
]}

WIELAND, FT ^{[1
]}

机构：

[1] MAX PLANCK INST BIOCHEM,GENZENTRUM,D-82152 MARTINSRIED,GERMANY

来源：

FEBS LETTERS | 1993年 / 332卷 / 1-2期

关键词：

SECRETORY PATHWAY; TRANSPORT VESICLE; COATOMER; YEAST HOMOLOG;

D O I：

10.1016/0014-5793(93)80487-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The homologue of the mammalian coatomer complex was isolated from yeast cytosol and separated on a modified urea-containing SDS-polyacrylamide gel system. An additional band in the 100 kDa molecular weight range appeared when compared to the protein pattern obtained in conventional Laemmli gels, exactly as observed for mammalian coatomer. Cross-reactivity with an anti-peptide antibody raised against the C-terminus of beta'-COP from bovine, and N-terminal sequence analysis, revealed that this protein from yeast is related to beta'-COP from mammals.

引用

页码：71 / 73

页数：3

共 24 条

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