LINEAR, NONMITOCHONDRIAL PLASMIDS OF ALTERNARIA-ALTERNATA

被引:8
作者
SHEPHERD, HS
机构
[1] ARS Southern Regional Research Center, United States Department of Agriculture, New Orleans, 70179, LA
关键词
ALTERNARIA; LINEAR PLASMIDS; FUNGAL PLASMIDS;
D O I
10.1007/BF00318477
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Three plasmids, with sizes of 7.0 kbp, 6.8 kbp, and 5.0 kbp and designated pAal-1, pAal-2 and pAal-3 respectively, have been found in a tentoxin-producing isolate of Alternaria alternata. Exonuclease digestions show these plasmids to be linear with blocked 5' ends. Plasmid pAal-1 does not hybridize to nuclear DNA, mitochondrial DNA, or double-stranded RNA from a mycovirus found in the isolate, but does hybridize weakly to a series of linear DNAs which are not visible on gels and may include pAal-2 and pAal-3. Cellular fractionation shows that, unlike other linear fungal plasmids, these plasmids are not localized in the mitochondria. Plasmids have not been found in other tentoxin-producing isolates and there is no evidence that these plasmids have any effect on the production of tentoxin.
引用
收藏
页码:169 / 172
页数:4
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