GENETIC AND PHYSICAL MAPPING OF AN HYDROGENASE GENE-CLUSTER FROM RHODOBACTER-CAPSULATUS

被引：40

作者：

COLBEAU, A ^{[1
]}

MAGNIN, JP ^{[1
]}

CAUVIN, B ^{[1
]}

CHAMPION, T ^{[1
]}

VIGNAIS, PM ^{[1
]}

机构：

[1] CEN,DEPT RECH FONDAMENTALE,BIOCHIM MICROBIENNE LAB,CNRS,UA 1130,85X,F-38041 GRENOBLE,FRANCE

来源：

MOLECULAR & GENERAL GENETICS | 1990年 / 220卷 / 03期

关键词：

(NiFe)hydrogenase; Chromosomal mapping; Hydrogenase (hup) genes; IS; 21; Rhodobacter capsulatus hupS; hupL;

D O I：

10.1007/BF00391744

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An hydrogenase-deficient (Hup-) mutant of Rhodobacter capsulatus was obtained by adventitious insertion of IS 21 DNA into an hydrogenase structural gene (hup) of the wild-type strain 1310. The resulting Hup- mutant, strain JP91, selected by its inability to grow autotrophically (Aut- phenotype) together with other Hup- mutant strains obtained by classical ethyl methane sulphonate mutagenesis were used in R plasmid-mediated conjugation experiments to map the hup/aut loci on the chromosome of R. capsulatus. The hup genes tested in this study were found to cluster on the chromosome in the proximity of the his-1 marker. A cluster of hup genes comprising the structural genes was isolated from a gene bank constructed in the cosmid vector pHC79 with 40 kb insert DNA. The clustered hup genes, characterized by hybridization studies and complementation analyses of the R. capsulatus Hup- mutants, span 15-20 kb of DNA. © 1990 Springer-Verlag.

引用

页码：393 / 399

页数：7

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