CAROTENOID-BIOSYNTHESIS GENES AS A GENETIC-MARKER FOR THE PURPOSE OF GENE CLONING

被引：2
作者：
LIU, ST
机构：
[1] Chang Gung Med. Coll., Department Microbiol and Immunol, Molec Genet Lab., Kwei Shan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 195卷 / 01期
关键词：
D O I：
10.1006/bbrc.1993.2038
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A cloning vector pSL775 (7.0 kb) was constructed using the carotenoid-biosynthesis genes of Erwinia herbicola Eho 13 (ATCC 53489). This vector contained a ColE1 origin, an ampicillin resistant gene, and a total of 11 single cloning sites: Asp718, Ava1, BamHI, BanII, EcoRV, HindIII, KpnI, MluI, NcoI, NotI, and SmaI. Transforming the vector into an Escherichia coli strain could result in pink clones. On the other hand, insertion of a DNA fragment into one of these cloning sites resulted in nonpigmented clones. The color differential between the two types of colonies could be detected visually on agar medium after culturing the cells at 37°C for 18 hours. © 1993 Academic Press. All rights reserved.
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页码：259 / 263
页数：5
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