AN ORDERED COLLECTION OF BACILLUS-SUBTILIS DNA SEGMENTS CLONED IN YEAST ARTIFICIAL CHROMOSOMES

被引：55
作者：
AZEVEDO, V
ALVAREZ, E
ZUMSTEIN, E
DAMIANI, G
SGARAMELLA, V
EHRLICH, SD
SERROR, P
机构：
[1] INRA,GENET MICROBIENNE LAB,DOMAINE VILVERT,F-78352 JOUY EN JOSAS,FRANCE
[2] SMITH KLINE BEECHAM PHARMACEUT,CTR INVEST BASICA,E-28760 TRES CANTOS,SPAIN
[3] CNR,IDVGA,I-20133 MILAN,ITALY
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1993年 / 90卷 / 13期
关键词：
PHYSICAL MAP; CONTIG; HYBRIDIZATION;
D O I：
10.1073/pnas.90.13.6047
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A collection of 772 Bacillus subtilis DNA segments was obtained by cloning in yeast artificial chromosomes. The B. subtilis inserts of 288 clones were mapped by hybridization using as probes 65 cloned genes and 188 isolated insert ends. In this way, 59 inserts were ordered in four contigs that cover >98% of the B. subtilis chromosome. This ordered collection is now available for further genetic and physical analysis of the B. subtilis genome.
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页码：6047 / 6051
页数：5
相关论文
共 35 条
[1]   CONSTRUCTION AND CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME LIBRARY CONTAINING 7 HAPLOID HUMAN GENOME EQUIVALENTS [J].
ALBERTSEN, HM ;
ABDERRAHIM, H ;
CANN, HM ;
DAUSSET, J ;
LEPASLIER, D ;
COHEN, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (11) :4256-4260
[2]  
AMJAD M, 1990, GENE, V101, P15
[3]  
Anagnostopoulos C., 1993, P425
[4]   CONSTRUCTION OF YEAST ARTIFICIAL CHROMOSOME LIBRARIES WITH LARGE INSERTS USING FRACTIONATION BY PULSED-FIELD GEL-ELECTROPHORESIS [J].
ANAND, R ;
VILLASANTE, A ;
TYLERSMITH, C .
NUCLEIC ACIDS RESEARCH, 1989, 17 (09) :3425-3433
[5]  
Ausubel F, 1988, CURRENT PROTOCOLS MO
[6]   TRANSFORMATION OF YEAST SPHEROPLASTS WITHOUT CELL-FUSION [J].
BURGERS, PMJ ;
PERCIVAL, KJ .
ANALYTICAL BIOCHEMISTRY, 1987, 163 (02) :391-397
[7]   CLONING OF LARGE SEGMENTS OF EXOGENOUS DNA INTO YEAST BY MEANS OF ARTIFICIAL CHROMOSOME VECTORS [J].
BURKE, DT ;
CARLE, GF ;
OLSON, MV .
SCIENCE, 1987, 236 (4803) :806-812
[8]   TOWARD A PHYSICAL MAP OF THE GENOME OF THE NEMATODE CAENORHABDITIS-ELEGANS [J].
COULSON, A ;
SULSTON, J ;
BRENNER, S ;
KARN, J .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (20) :7821-7825
[9]   GENOME LINKING WITH YEAST ARTIFICIAL CHROMOSOMES [J].
COULSON, A ;
WATERSTON, R ;
KIFF, J ;
SULSTON, J ;
KOHARA, Y .
NATURE, 1988, 335 (6186) :184-186
[10]   YACS AND THE C-ELEGANS GENOME [J].
COULSON, A ;
KOZONO, Y ;
LUTTERBACH, B ;
SHOWNKEEN, R ;
SULSTON, J ;
WATERSTON, R .
BIOESSAYS, 1991, 13 (08) :413-417
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