TRANSGENIC PLANTS OF TALL FESCUE (FESTUCA-ARUNDINACEA SCHREB) OBTAINED BY DIRECT GENE-TRANSFER TO PROTOPLASTS

被引：99

作者：

WANG, ZY ^{[1
]}

TAKAMIZO, T ^{[1
]}

IGLESIAS, VA ^{[1
]}

OSUSKY, M ^{[1
]}

NAGEL, J ^{[1
]}

POTRYKUS, I ^{[1
]}

SPANGENBERG, G ^{[1
]}

机构：

[1] SWISS FED INST TECHNOL,INST PLANT SCI,CH-8092 ZURICH,SWITZERLAND

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 06期

关键词：

D O I：

10.1038/nbt0692-691

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Chimeric hygromycin phosphotransferase (hph) and phosphinothricin acetyltransferase (bar) genes were introduced, using polyethylene glycol treatment, into protoplasts isolated from embryogenic cell suspension cultures of tall fescue (Festuca arundinacea Schreb.), a graminaceous plant that is an important forage crop in temperate pastures. Colonies resistant to either 200 mg/l hygromycin or 100 mg/l phosphinothricin, respectively, were recovered upon selection using bead-type culture systems. Stable integration of the transgenes in the genomes of plants regenerated from resistant callus clones was shown by Southern hybridization analysis. In situ hybridization of a labeled transgene-probe to metaphase chromosomes is shown for one transgenic primary regenerant. Expression of the transgenes in mature plants was demonstrated by HPH enzyme assay or by phosphinothricin-herbicide spraying.

引用

页码：691 / 699

页数：9

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