RECONSTITUTION OF THE SOLUBILIZED INSULIN-RECEPTOR IN PHOSPHOLIPID-VESICLES

被引：20
作者：
GOULD, RJ
GINSBERG, BH
SPECTOR, AA
机构：
[1] VET ADM HOSP,IOWA CITY,IA 52242
[2] UNIV IOWA,DEPT INTERNAL MED,IOWA CITY,IA 52242
[3] UNIV IOWA,DIABET ENDOCRINOL RES CTR,IOWA CITY,IA 52242
来源：
ENDOCRINE RESEARCH COMMUNICATIONS | 1979年 / 6卷 / 04期
关键词：
D O I：
10.1080/07435807909061107
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The insulin receptor was solubilized from turkey erythrocyte membranes by extraction with 1% βoctylglucopyranoside. Insulin binding was enhanced when the solubilized material was reconstituted in phospholipid vesicles. The affinity of the reconstituted vesicles for various insulins was similar to that of the intact membranes: porcine insulin > proinsulin > desoctapeptide insulin. A curvilinear Scatchard plot was obtained for insulin binding to the reconstituted system at 15d̀C. A high affinity association constant of 1.4 X 109 M-1 was obtained from the Scatchard plot. This is a four-fold increase over the value for the turkey erythrocyte membrane, which contains more highly saturated phospholipids. This suggests that the insulin receptor may be sensitive to the lipid composition of the membranes in which it is embedded. © 1979 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.
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页码：279 / 290
页数：12
相关论文
共 13 条
[1]  
BRENNEMAN DE, 1977, J LIPID RES, V18, P582
[2]   SINGLE BILAYER VESICLES PREPARED WITHOUT SONICATION PHYSICOCHEMICAL PROPERTIES [J].
BRUNNER, J ;
SKRABAL, P ;
HAUSER, H .
BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 455 (02) :322-331
[3]   RECONSTITUTION OF PURIFIED DETERGENT-SOLUBLE HLA-A AND HLA-B ANTIGENS INTO PHOSPHOLIPID VESICLES [J].
ENGELHARD, VH ;
GUILD, BC ;
HELENIUS, A ;
TERHORST, C ;
STROMINGER, JL .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (07) :3230-3234
[4]   PROPERTIES AND PARTIAL-PURIFICATION OF DETERGENT-SOLUBILIZED INSULIN RECEPTOR - DEMONSTRATION OF NEGATIVE COOPERATIVITY IN MICELLAR SOLUTION [J].
GINSBERG, BH ;
COHEN, RM ;
KAHN, CR ;
ROTH, J .
BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 542 (01) :88-100
[5]   INSULIN RECEPTOR OF TURKEY ERYTHROCYTE CHARACTERIZATION OF MEMBRANE-BOUND RECEPTOR [J].
GINSBERG, BH ;
KAHN, CR ;
ROTH, J .
BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 443 (02) :227-242
[6]  
GINSBERG BH, 1977, BIOCHEM ACTION HORM, V4, P313
[7]   MEMBRANE RECEPTORS FOR HORMONES AND NEUROTRANSMITTERS [J].
KAHN, CR .
JOURNAL OF CELL BIOLOGY, 1976, 70 (02) :261-286
[8]  
LOWRY OH, 1951, J BIOL CHEM, V193, P265
[9]   INFLUENCE OF LIPID SUBSTITUTION ON THYROTROPIN-RECEPTOR INTERACTIONS IN ARTIFICIAL VESICLES [J].
MEHDI, SQ ;
NUSSEY, SS ;
SHINDELMAN, JE ;
KRISS, JP .
ENDOCRINOLOGY, 1977, 101 (05) :1406-1412
[10]  
PETRI WA, 1979, J BIOL CHEM, V254, P4313
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