DYNAMICS IN SYNTHETIC OLIGONUCLEOTIDES - A SOLID-STATE DEUTERIUM NMR-STUDY

被引：20

作者：

ALAM, TM ^{[1
]}

DROBNY, G ^{[1
]}

机构：

[1] UNIV WASHINGTON,DEPT CHEM,SEATTLE,WA 98195

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 14期

关键词：

D O I：

10.1021/bi00466a001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Solid-state 2H NMR spectroscopy was used to investigate dynamics in the [methyl-2H]thy-midine-labeled oligonucleotide [d(CGCGAAT*T*CGCG)]2. Quadrupole echo line shapes, spin-lattice relaxation, and quadrupolar echo decay rates were investigated as a function of hydration W (moles of water/moles of nucleotide) between 0 and ~30. The amplitude of the base motion, modeled as a fast four-site libration, or diffusion in a cone, increased slightly with higher levels of hydration. A slower component of motion about the helix axis appears at W ~ 10 and increases in rate and amplitude, leading to the intermediate rate line shape observed at W ~ 21. © 1990, American Chemical Society. All rights reserved.

引用

页码：3421 / 3430

页数：10

共 77 条

[1]

Abragam A., 1961, PRINCIPLES NUCLEAR M, P216

[2]

ALAM TM, 1990, IN PRESS J CHEM PHYS

[3] TORSION DYNAMICS AND DEPOLARIZATION OF FLUORESCENCE OF LINEAR MACROMOLECULES .1. THEORY AND APPLICATION TO DNA [J].