QUANTITATIVE ESTIMATION OF PROTOZOA FROM SANDY SUBSTRATES

被引：16

作者：

DYE, AH

机构：

[1] Department of Zoology, University of Port Elizabeth, Port Elizabeth, 6000

来源：

ESTUARINE AND COASTAL MARINE SCIENCE | 1979年 / 8卷 / 02期

关键词：

fluorescence; protozoa; sand organisms; South Africa coast;

D O I：

10.1016/0302-3524(79)90075-6

中图分类号：

Q17 [水生生物学];

学科分类号：

071004 ;

摘要：

A rapid method for the quantitative estimation of protozoa populations in sand is described and compared with the seawater-ice technique. The method involves the gentle shaking of sand samples in filtered seawater and staining with Acridine Orange for counting under a fluorescence microscope. Apart from the fluorescence equipment no specialized apparatus is required and a large number of samples may be treated simultaneously. Since Acridine Orange is not a vital stain pre-fixing in 5% formalin is possible enabling samples to be stored prior to extraction. The results of a study of the distribution and abundance of protozoa on two beaches in the eastern Cape Province of South Africa are presented as an illustration of the method. © 1979.

引用

页码：199 / 204

页数：6

共 21 条

[1] ECOLOGY OF 2 SANDY BEACHES IN SOUTH WEST INDIA .2. NOTES ON EMERITA-HOLTHUISI [J].