THE N-TERMINUS OF AMINE OXIDASE OF HANSENULA-POLYMORPHA CONTAINS A PEROXISOMAL TARGETING SIGNAL

被引：49
|
作者：
FABER, KN
KEIZERGUNNINK, I
PLUIM, D
HARDER, W
AB, G
VEENHUIS, M
机构：
[1] UNIV GRONINGEN,CTR BIOL,DEPT MICROBIOL,9751 NN HAREN,NETHERLANDS
[2] UNIV GRONINGEN,BIOCHEM LAB,9747 AG GRONINGEN,NETHERLANDS
来源：
FEBS LETTERS | 1995年 / 357卷 / 02期
关键词：
PEROXISOME; TOPOGENIC SIGNAL; AMINE OXIDASE; PTS2; BETA-LACTAMASE; WATERMELON MALATE DEHYDROGENASE;
D O I：
10.1016/0014-5793(94)01317-T
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Here we describe the identification of the targeting sequence of peroxisomal amine oxidase (AMO) of H. polymorpha. Deletion analysis revealed that essential targeting information is located within the extreme N-terminal 16 amino acids. Moreover, this sequence can direct a reporter protein to the peroxisomal matrix of H. polymorpha. The N-terminal 16 amino acids of AMO contain a sequence with strong homology to the conserved PTS2 sequence. Therefore, AMO is considered to be a PTS2 protein.
引用
收藏
页码：115 / 120
页数：6
相关论文
共 50 条
  • [1] THE PEROXISOMAL IMPORT SIGNAL OF AMINE OXIDASE FROM THE YEAST HANSENULA-POLYMORPHA IS NOT UNIVERSAL
    DEHOOP, MJ
    VALKEMA, R
    KIENHUIS, CBM
    HOYER, MA
    AB, G
    YEAST, 1992, 8 (04) : 243 - 252
  • [2] CLONING AND SEQUENCING OF THE PEROXISOMAL AMINE OXIDASE GENE FROM HANSENULA-POLYMORPHA
    BRUINENBERG, PG
    EVERS, M
    WATERHAM, HR
    KUIPERS, J
    ARNBERG, AC
    AB, G
    BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1008 (02) : 157 - 167
  • [3] TARGETING SIGNAL OF THE PEROXISOMAL CATALASE IN THE METHYLOTROPHIC YEAST HANSENULA-POLYMORPHA
    DIDION, T
    ROGGENKAMP, R
    FEBS LETTERS, 1992, 303 (2-3) : 113 - 116
  • [4] PEROXISOMAL AMINE OXIDASE OF HANSENULA-POLYMORPHA DOES NOT REQUIRE ITS SRL-CONTAINING C-TERMINAL SEQUENCE FOR TARGETING
    FABER, KN
    HAIMA, P
    DEHOOP, MJ
    HARDER, W
    VEENHUIS, M
    AB, G
    YEAST, 1993, 9 (04) : 331 - 338
  • [5] BIOSYNTHESIS AND REGULATION OF THE PEROXISOMAL METHANOL OXIDASE FROM THE METHYLOTROPHIC YEAST HANSENULA-POLYMORPHA
    ROGGENKAMP, R
    JANOWICZ, Z
    STANIKOWSKI, B
    HOLLENBERG, CP
    MOLECULAR AND GENERAL GENETICS, 1984, 194 (03): : 489 - 493
  • [6] THE METHYLOTROPHIC YEAST HANSENULA-POLYMORPHA CONTAINS AN INDUCIBLE IMPORT PATHWAY FOR PEROXISOMAL MATRIX PROTEINS WITH AN N-TERMINAL TARGETING SIGNAL (PTS2 PROTEINS)
    FABER, KN
    HAIMA, P
    GIETL, C
    HARDER, W
    AB, G
    VEENHUIS, M
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (26) : 12985 - 12989
  • [7] INVIVO INACTIVATION OF PEROXISOMAL ALCOHOL OXIDASE IN HANSENULA-POLYMORPHA BY KCN IS AN IRREVERSIBLE PROCESS
    VANDERKLEI, IJ
    VEENHUIS, M
    NICOLAY, K
    HARDER, W
    ARCHIVES OF MICROBIOLOGY, 1989, 151 (01) : 26 - 33
  • [8] TARGETING SEQUENCES OF THE 2 MAJOR PEROXISOMAL PROTEINS IN THE METHYLOTROPHIC YEAST HANSENULA-POLYMORPHA
    HANSEN, H
    DIDION, T
    THIEMANN, A
    VEENHUIS, M
    ROGGENKAMP, R
    MOLECULAR & GENERAL GENETICS, 1992, 235 (2-3): : 269 - 278
  • [9] INVIVO INACTIVATION OF ALCOHOL OXIDASE IN HANSENULA-POLYMORPHA
    VANDERKLEI, IJ
    VEENHUIS, M
    HARDER, W
    ULTRAMICROSCOPY, 1988, 24 (04) : 439 - 439
  • [10] BIOSYNTHESIS OF PEROXISOMAL ENZYMES IN THE METHYLOTROPHIC YEAST HANSENULA-POLYMORPHA
    ROA, M
    BLOBEL, G
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (22): : 6872 - 6876
12345