IDENTIFICATION OF O-HYDROXYPHENYLACETALDEHYDE AS A MAJOR METABOLITE OF COUMARIN IN RAT HEPATIC MICROSOMES

被引：28

作者：

LAKE, BG ^{[1
]}

OSBORNE, DJ ^{[1
]}

WALTERS, DG ^{[1
]}

PRICE, RJ ^{[1
]}

机构：

[1] LILLY RES CTR LTD,WINDLESHAM GU20 6PH,SURREY,ENGLAND

来源：

FOOD AND CHEMICAL TOXICOLOGY | 1992年 / 30卷 / 02期

关键词：

D O I：

10.1016/0278-6915(92)90144-A

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The metabolism of [3-C-14]coumarin has been studied in hepatic microsomes from control (corn-oil treated) and Aroclor 1254-treated (100 mg/kg body weight/day, 5 days, ip) rats. [3-C-14]Coumarin metabolites in incubate extracts were separated by HPLC and identified by comparison with the retention times of known coumarin metabolites. The major product produced by incubation of 0.25-2.5 mM-[3-C-14]coumarin with both control and Aroclor 1254-induced hepatic microsomes was a novel coumarin metabolite. This novel metabolite was extracted from pooled microsomal incubations, purified by semi-preparative HPLC and identified by mass spectrometry as o-hydroxyphenylacetaldehyde (o-HPA). Some possible pathways for the formation of o-HPA from coumarin are proposed.

引用

页码：99 / 104

页数：6

共 22 条

[1] CRITICAL-REVIEW OF THE TOXICOLOGY OF COUMARIN WITH SPECIAL REFERENCE TO INTERSPECIES DIFFERENCES IN METABOLISM AND HEPATOTOXIC RESPONSE AND THEIR SIGNIFICANCE TO MAN [J].