OPTIMIZED SOLVENT PROGRAMMING FOR SEPARATIONS OF COMPLEX SAMPLES BY LIQUID-SOLID ADSORPTION CHROMATOGRAPHY IN COLUMNS

被引：124

作者：

SNYDER, LR

SAUNDERS, DL

机构：

[1] Union OH Company of California, Research Department, Brea, CA, 92621

[2] Fifth International Symposium on Advances in Chromatography held in Las Vegas, NV

来源：

JOURNAL OF CHROMATOGRAPHIC SCIENCE | 1969年 / 7卷 / 04期

关键词：

D O I：

10.1093/chromsci/7.4.195

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In most chromatographic procedures, the efficient separation of a complex mixture requires a systematic change in sample distribution coefficients (K values) during separation. In the case of liquid-solid column chromatography, this is best accomplished by some form of solvent programming (stepwise or gradient elution). The present paper provides a theoretical derivation of sample resolution in solvent programmed systems as a function of the solvent program and other experimental variables. Conditions for optimum sample resolution per unit time are discussed. The application of these results to specific problems is straightforward and simple. Various other aspects of optimized solvent programming are also discussed. Some experimental examples of high resolution separation by solvent-programmed liquid-solid chromatography are shown. © 1969, Oxford University Press. All rights reserved.

引用

页码：195 / &

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