COLONY FORMATION FROM PROTOPLASTS DERIVED FROM DOUGLAS-FIR COTYLEDONS

被引：34

作者：

KIRBY, EG ^{[1
]}

CHENG, TY ^{[1
]}

机构：

[1] OREGON GRAD CTR STUDY & RES,DEPT CHEM BIOCHEM SCI,BEAVERTON,OR 97005

来源：

PLANT SCIENCE LETTERS | 1979年 / 14卷 / 02期

关键词：

D O I：

10.1016/0304-4211(79)90053-1

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Proliferation of Douglas fir cells regenerated from protoplasts has been accomplished using cotyledons derived from 2-4 week old seedlings. Yield of protoplasts was significantly increased by subjecting cotyledons, prior to protoplast isolation, to a preconditioning treatment for 8 days in the presence of 15 mu;M N6-benzylaminopurine (BAP) and 500 nM 2-naphthaleneacetic acid (NAA). Protoplasts cultured on a fabric tissue support saturated with a liquid nutrient medium containing a high concentration (10 mM) of glutamine were capable of undergoing rapid cell division leading to colony formation. © 1979.

引用

页码：145 / 154

页数：10

共 10 条

[1] REGENERATION OF DOUGLAS-FIR PLANTLETS THROUGH TISSUE-CULTURE [J].

CHENG, TY ;

VOQUI, TH .

SCIENCE, 1977, 198 (4314) :306-307

[2] FACTORS EFFECTING ADVENTITIOUS BUD FORMATION OF COTYLEDON CULTURE OF DOUGLAS-FIR [J].

CHENG, TY .

PLANT SCIENCE LETTERS, 1977, 9 (02) :179-187

[3] CULTURE OF PROTOPLASTS DERIVED FROM RAMSEY DURUM-WHEAT [J].

FARMER, I ;

LEE, PE .

PLANT SCIENCE LETTERS, 1977, 10 (02) :141-145

[4] LEAF PRETREATMENT WITH SENESCENCE RETARDANTS AS A BASIS FOR OAT PROTOPLAST IMPROVEMENT [J].

KAURSAWHNEY, R ;

ADAMS, WR ;

TSANG, J ;

GALSTON, AW .

PLANT AND CELL PHYSIOLOGY, 1977, 18 (06) :1309-1317

[5] EFFECT OF CYCLOHEXIMIDE AND KINETIN ON YIELD, INTEGRITY AND METABOLIC-ACTIVITY OF OAT LEAF PROTOPLASTS [J].

KAURSAWHNEY, R ;

RANCILLAC, M ;

STASKAWICZ, B ;

ADAMS, WR ;

GALSTON, AW .

PLANT SCIENCE LETTERS, 1976, 7 (01) :57-67

[6] CELL WALL REGENERATION AND CELL DIVISION IN ISOLATED TOBACCO MESOPHYLL PROTOPLASTS [J].

NAGATA, T ;

TAKEBE, I .

PLANTA, 1970, 92 (04) :301-&

[7]

POTRYKUS L, 1977, MOL GEN GENET, V156, P347

[8] MEMBRANE PROPERTIES OF LIVING MAMMALIAN CELLS AS STUDIED BY ENZYMATIC HYDROLYSIS OF FLUOROGENIC ESTERS [J].

ROTMAN, B ;

PAPERMASTER, BW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1966, 55 (01) :134-+

[9]

Skene K. G. M., 1975, Vitis, V14, P177

[10] PRETREATMENT OF CELL-SUSPENSIONS AS A METHOD TO INCREASE PROTOPLAST YIELD OF HAPLOPAPPUS-GRACILIS [J].

WALLIN, A ;

GLIMELIUS, K ;

ERIKSSON, T .

PHYSIOLOGIA PLANTARUM, 1977, 40 (04) :307-311

← 1 →