DISTRIBUTION OF LHRH-LIKE SUBSTANCE IN THE VERTEBRATE BRAIN AS REVEALED BY IMMUNOHISTOCHEMISTRY

被引:107
作者
NOZAKI, M [1 ]
KOBAYASHI, H [1 ]
机构
[1] TOHO UNIV,FAC SCI,DEPT BIOL,FUNABASHI,CHIBA 274,JAPAN
来源
ARCHIVUM HISTOLOGICUM JAPONICUM | 1979年 / 42卷 / 03期
关键词
D O I
10.1679/aohc1950.42.201
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Distribution of immunoreactive LHRH-like substance was studied by PAP method using two different anti-LHRH sera (AsI: anti-Tyr5-BSA-LHRH serum; AsII: anti-Gly10-BSA-LHRH serum) in the brain of several species selected from each class of vertebrates. Although LHRH-immunoreactivity was slightly stronger in AsI than AsII, LHRH-like substance was found in the median eminence of all tetrapods tested (Table 1). LHRH-positive perikarya were detected by both Asl and AsII primarily in the septo-preoptic region of the cat, Elaphe climacophora, Rana catesbeiana, Xenopus laevis, Cynopus pyrrhogaster ensicauda. However, in the guinea pig, those perikarya were detected only by Asl in the medial basal hypothalamic region in addition to the septo-preoptic region. In most of those animals, LHRH-positive fiber pathways to the median eminence were also demonstrated. Among the teleosts examined, immunoreactive products were detectable in the fiber terminals of the neurohypophysis of Anguilla japonica and Fugu niphobles, butnot in the three other species (Table 1). In the former two species, however, LHRH-immunoreactivity was slightly stronger in Asl than AsII. When Asl was applied to the hypothalamic sections of the lamprey, Entospenus japonica, dense immunoreactive product was found in the nerve perikarya of the preoptic nucleus and in the fiber terminals of the neurohypophysis. However, when AsII was applied to the lamprey, only a little immunoreactive product was detectable in the same regions. No immunoreactive product was detected in the brain of the dogfish (Triakis scyllia) and the hagfish (Eptatretus burgeri) in either case of Asl or AsII. © 1979, International Society of Histology and Cytology. All rights reserved.
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页码:201 / 219
页数:19
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