High-level production and characterization of lactase from a newly isolated fungus Trametes sp. LS-10C

被引：28

作者：

Li, Song ^{[1
,2
]}

Tang, Bin ^{[1
,2
]}

Liu, Yu ^{[1
,2
]}

Chen, Ana ^{[1
,2
]}

Tang, Wenjing ^{[1
,2
]}

Wei, Shenghua ^{[1
,2
]}

机构：

[1] Anhui Polytech Univ, Microorganism Fermentat Engn & Technol Res Ctr An, Cent Beijing Rd, Wuhu 241000, Peoples R China

[2] Anhui Polytech Univ, Sch Biol & Chem Engn, Cent Beijing Rd, Wuhu 241000, Peoples R China

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2016年 / 8卷

基金：

中国国家自然科学基金;

关键词：

Trametes sp; Lactase; Fermentation; Optimization; Characterization;

D O I：

10.1016/j.bcab.2016.10.008

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An efficient lactase production fungus Trametes sp. LS-10C was isolated and identified, and the lactase producing medium was optimized by response surface methodology (RSM) in shake flask fermentation. The highest lactase activity obtained at 9 d in shake flask and 10-L fermentator were 588.61 +/- 2.2 U mL(-1) and 873.82 +/- 22.91 U mL(-1), respectively. The molecular weight of purified lactase determined by SDS-PAGE was around 65 kDa. The optimal pH was 4.0-4.5 and the optimal temperature was 40 degrees C. Among the tested substrates of ABTS, DMP and guaiacol, both the highest values of V-max (11.4 +/- 1.5 mM min(-1)) and k(cat)/K-m, (6653.2 +/- 18.2 mM(-1) s(-1)) were found for ABTS and the lowest K-m value (38.1 +/- 1.7 mu m) was found for DMP.

引用

页码：278 / 285

页数：8

共 35 条

[21] Development of a Saccharomyces cerevisiae strain with enhanced resistance to phenolic fermentation inhibitors in lignocellulose hydrolysates by heterologous expression of laccase [J].