REGULATION OF NICOTINE-EVOKED DOPAMINE RELEASE FROM PC12 CELLS

被引：11
作者：
COURTNEY, ND
HOWLETT, AC
WESTFALL, TC
机构：
[1] ST LOUIS UNIV,SCH MED,DEPT PHARMACOL & PHYSIOL SCI,ST LOUIS,MO 63104
[2] VET ADM MED CTR,RES SERV 151E,PORTLAND,OR 97201
来源：
LIFE SCIENCES | 1991年 / 48卷 / 17期
关键词：
D O I：
10.1016/0024-3205(91)90127-W
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The nature of second messengers involved in the nicotine-evoked release of dopamine from PC12 cells was examined. Calmidazolium, a calmodulin inhibitor, abolished the nicotine-evoked release. A23187, a Ca2+ ionophore, enhanced dopamine release, and this was inhibited by calmidazolium. Further, 2', 5'-dideoxyadenosine abolished both the nicotine- and A23187-evoked release. Forskolin, dibutyryl-cyclic AMP, and rolipram (a cyclic AMP phosphodiesterase inhibitor) all enhanced dopamine release. 1, 9-Dideoxyforskolin, a forskolin analog which does not activate adenylate cyclase, did not alter dopamine release. These results suggest an obligatory role for Ca2+ and calmodulin-sensitive adenylate cyclase in the nicotine-evoked release process.
引用
收藏
页码：1671 / 1678
页数：8
相关论文
共 34 条
[31]  
STANDAERT FG, 1979, FED PROC, V38, P2183
[32]   TUMOR PROMOTER PHORBOL 12-MYRISTATE, 13-ACETATE INHIBITS PHOSPHOINOSITIDE HYDROLYSIS AND CYTOSOLIC CA-2+ RISE INDUCED BY THE ACTIVATION OF MUSCARINIC RECEPTORS IN PC12 CELLS [J].
VICENTINI, LM ;
DIVIRGILIO, F ;
AMBROSINI, A ;
POZZAN, T ;
MELDOLESI, J .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1985, 127 (01) :310-317
[33]  
WESTFALL TC, 1976, J PHARMACOL EXP THER, V199, P149
[34]  
WHITE MM, 1988, MOL PHARMACOL, V34, P427
1234