HALOCYAMINES - NOVEL ANTIMICROBIAL TETRAPEPTIDE-LIKE SUBSTANCES ISOLATED FROM THE HEMOCYTES OF THE SOLITARY ASCIDIAN HALOCYNTHIA-RORETZI

被引：116

作者：

AZUMI, K ^{[1
]}

YOKOSAWA, H ^{[1
]}

ISHII, S ^{[1
]}

机构：

[1] HOKKAIDO UNIV, FAC PHARMACEUT SCI, DEPT BIOCHEM, SAPPORO, HOKKAIDO 060, JAPAN

来源：

BIOCHEMISTRY | 1990年 / 29卷 / 01期

关键词：

D O I：

10.1021/bi00453a021

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two novel antimicrobial tetrapeptide-like substances, halocyamine A and B, were isolated from the solitary ascidian Halocynthia roretzi by a procedure including extraction steps, chromatographies on coarse and fine HP-20 columns, and preparative reversed-phase high-performance liquid chromatography. The structures of halocyamine A and B were determined to be l-histidyl-l-6,7-dihydroxyphenylalanyl-glycyl-6-bromo-8,9-didehydrotryptamine and l-threonyl-l-6,7-dihydroxyphenylalanyl-l-histidyl-6-bromo-8,9-didehydrotryptamine, respectively, by spectral analyses and degradation studies. Besides antimicrobial activities against several kinds of bacteria and yeasts, both of them showed cytotoxic activities against neuronal cells cultured from rat fetal brain, mouse neuroblastoma N-18 cells, and human hepatoma Hep-G2 cells. They were only detected in the “morula”-like cells, which are of the most abundant cell type among the hemocytes of H. roretzi. © 1990, American Chemical Society. All rights reserved.

引用

页码：159 / 165

页数：7

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