DETECTION OF SUPEROXIDE ANION FROM THE REACTION OF OXYHEMOGLOBIN AND PHENYLHYDRAZINE USING EPR SPECTROSCOPY

被引：17

作者：

GOLDBERG, B

STERN, A

PEISACH, J

BLUMBERG, WE

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MOLEC PHARMACOL,BRONX,NY 10461

[2] YESHIVA UNIV ALBERT EINSTEIN COLL MED,DEPT MOLEC BIOL,BRONX,NY 10461

[3] BELL TEL LABS INC,MURRAY HILL,NJ 07974

来源：

EXPERIENTIA | 1979年 / 35卷 / 04期

关键词：

D O I：

10.1007/BF01922723

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The low temperature EPR spectrum of a quickly reacted mixture of oxyhemoglobin and phenylhydrazine was studied. With the use of a computer, the spectral contribution of methemoglobin in the region of g=2 was subtracted. The remaining spectrum was that of an axial free radical (g⊥=2.00, g{norm of matrix}=2.06) having the magnetic parameters of superoxide anion. In the presence of superoxide dismutase, this axial radical was not seen, confirming that superoxide anion is indeed generated by the reaction. © 1979 Birkhäuser Verlag.

引用

页码：488 / 489

页数：2

共 7 条

[1]

BERZOFSKY JA, 1971, J BIOL CHEM, V246, P3367

[2]

FEHER G, 1956, BELL SYST TECH J, V26, P449

[3]

GOLDBERG B, 1975, J BIOL CHEM, V250, P2401

[4]

GOLDBERG B, 1976, J BIOL CHEM, V251, P3045