APPLICATION OF STAINS-ALL STAINING TO THE ANALYSIS OF AXONEMAL TUBULINS - IDENTIFICATION OF BETA-TUBULIN AND BETA-ISOTUBULINS

被引：8

作者：

NAKAMURA, K ^{[1
]}

MASUYAMA, E ^{[1
]}

WADA, S ^{[1
]}

OKUNO, M ^{[1
]}

机构：

[1] UNIV TOKYO,COLL ARTS & SCI,DEPT BIOL,TOKYO 113,JAPAN

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1990年 / 21卷 / 03期

关键词：

Axoneme; Isoelectric focusing; SDS-polyacrylamide gel electrophoresis; Stains-all; Tubulin;

D O I：

10.1016/0165-022X(90)90017-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The cationic dye, Stains-all, is known to stain brain β-tubulin blue and α-tubulin red (Serrano, L. et al. (1986) J. Biochem. Biophys. Methods 12, 281-287; Serrano, L. et al. (1989) Biochem. Int., 19, 235-246). The present experiments show that this stain can also be applied to detect β-tubulin in axonemal tubulins from various sources such as cilia of protozoa, sperm flagella of echinoderm, and sperm flagella of mollusc. Furthermore, these experiments showed that it selectively stains isoforms of axonemal β-tubulin blue following isoelectric focusing, whereas those of α-tubulin are stained red. These results indicate that Stains-all staining is a useful tool for electrophoretic analysis of axonemal tubulins. © 1990.

引用

页码：237 / 245

页数：9

共 24 条

[1] SEQUENCE OF ONE ALPHA-TUBULIN AND 2 BETA-TUBULIN GENES OF TETRAHYMENA-PYRIFORMIS - STRUCTURAL AND FUNCTIONAL-RELATIONSHIPS WITH OTHER EUKARYOTIC TUBULIN GENES [J].