MUSCARINIC RECEPTOR-MEDIATED INCREASE IN EXTRACELLULAR INOSITOL 1,4,5-TRISPHOSPHATE LEVELS IN THE RAT HIPPOCAMPUS - AN IN-VIVO MICRODIALYSIS STUDY

被引:0
作者
MINISCLOU, C [1 ]
ROUQUIER, L [1 ]
BENAVIDES, J [1 ]
SCATTON, B [1 ]
CLAUSTRE, Y [1 ]
机构
[1] SYNTHELABO RECH,LERS,DEPT BIOL,F-92220 BAGNEUX,FRANCE
关键词
INOSITOL 1,4,5-TRISPHOSPHATE; HIPPOCAMPUS; RAT; MICRODIALYSIS; MUSCARINIC RECEPTOR;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The extracellular concentration of inositol 1,4,5-trisphosphate (IP3) has been monitored in the ventral hippocampus of the anesthetized rat by using a microdialysis technique coupled to a radioreceptor assay. Three hours after the implantation of the cannula, basal extracellular concentration of IP3 (corrected for a 9% recovery) was 71 nM (0.39 pmol/60-mu l fraction) and remained stable for at least 5 h. Local infusion of carbachol for 60 min caused a significant concentration-related increase in extracellular IP3 levels (0, 24, and 57% at 1, 50, and 100 mu M, respectively). Acetylcholine (100 mu M) and muscarine (100 mu M) increased IP3 outflow by 40 and 42%, respectively. The effect of carbachol was fully prevented by coinfusion of 10 mu M pirenzepine and reduced by 1 mu M tetrodotoxin indicating that the carbachol response is mediated by neuronal muscarinic receptors. These data demonstrate the feasibility of using microdialysis and a radioreceptor assay to measure IP3 in the extracellular space. This approach could prove useful for the study of the in vivo operation of muscarinic and, by extension, a number of receptors coupled to phosphoinositide turnover.
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页码:557 / 562
页数:6
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