TRANSCOBALAMIN-II - COBALAMIN BINDING-SITES ARE PRESENT ON RABBIT GERM-CELLS

被引：6
作者：
BOUKHZER, E
ENNYA, A
FELDEN, F
GERARD, A
NEXO, E
NICOLAS, JP
GERARD, H
GUEANT, JL
机构：
[1] FAC MED VANDOEUVRE NANCY, INSERM, U308, BIOCHIM NUTR LAB, F-54500 VANDOEUVRE LES NANCY, FRANCE
[2] FAC MED VANDOEUVRE NANCY, HISTOL EMBRYOL LAB 2, VANDOEUVRE LES NANCY, FRANCE
[3] AARHUS UNIV HOSP, DK-8000 AARHUS, DENMARK
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1175卷 / 01期
关键词：
TRANSCOBALAMIN; TRANSCOBALAMIN-II RECEPTOR; COBALAMIN; ENDOCYTOSIS; (GERM CELL);
D O I：
10.1016/0167-4889(92)90019-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Specific binding sites for rabbit transcobalamin 11 have been found on isolated adult rabbit germ cells. Scatchard analysis revealed a single class of binding sites for [Co-57]cyanocobalamin-transcobalamin II with an association constant (K(a)) of 1.3 . 10(10) M-1 and 700 sites per cell. Binding was reversible, saturable and calcium dependent. Electron microscope radioautography following incubation with iodinated transcobalamin II at 4-degrees-C led to a detectable labeling mainly restricted to the plasma membrane.
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收藏
页码：128 / 131
页数：4
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