ENANTIOSELECTIVE ASSAY OF WARFARIN IN BLOOD-PLASMA BY LIQUID-CHROMATOGRAPHY ON CHIRALCEL OC

被引：8

作者：

ANDERSEN, C ^{[1
]}

BALMER, K ^{[1
]}

LAGERSTROM, PO ^{[1
]}

机构：

[1] ASTRA HASSLE AB,BIOANALYT CHEM,S-43183 MOLNDAL,SWEDEN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1993年 / 615卷 / 01期

关键词：

D O I：

10.1016/0378-4347(93)80303-L

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A stereoselective method for the two enantiomers of warfarin has been developed. Warfarin is extracted into dichloromethane-hexane from blood plasma. After concentration by evaporation, the enantiomers are resolved by liquid chromatography on Chiralcel OC employing a non-polar mobile phase. By fluorometric detection, concentrations in plasma down to 80 nmol/l (25 ng/ml) can be determined with a coefficient of variation of less than 15%. At the 200 nmol/l level, a coefficient of variation of ca. 4% was found.

引用

页码：159 / 163

页数：5

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