HAS NEGATIVE STAINING STILL A PLACE IN BIOMACROMOLECULAR ELECTRON-MICROSCOPY

被引：73
作者：
BREMER, A
HENN, C
ENGEL, A
BAUMEISTER, W
AEBI, U
机构：
[1] MAX PLANCK INST BIOCHEM, DEPT STRUCT BIOL, W-8033 MARTINSRIED, GERMANY
[2] JOHNS HOPKINS UNIV, SCH MED, DEPT CELL BIOL & ANAT, BALTIMORE, MD 21205 USA
来源：
ULTRAMICROSCOPY | 1992年 / 46卷 / 1-4期
关键词：
D O I：
10.1016/0304-3991(92)90008-8
中图分类号：
TH742 [显微镜];
学科分类号：
摘要：
Transmission electron microscopy of proteins has provided molecular- and in a few cases near-atomic-resolution structural information. In this review, we critically evaluate the potential and the limitations in obtaining molecular resolution, particularly with negatively stained specimens, and put these into perspective with cryomicroscopy of unstained frozen-hydrated and sugar-embedded preparations.
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页码：85 / 111
页数：27
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