GAS-DIFFUSION DILUTION FLOW-INJECTION METHOD FOR THE DETERMINATION OF ETHANOL IN BEVERAGES WITHOUT SAMPLE PRETREATMENT

被引：47
作者：
KUNNECKE, W
SCHMID, RD
机构：
[1] GBF - Gesellschaft für Biotechnologische Forschung, Department of Enzyme Technology, D-3300 Braunschweig
来源：
ANALYTICA CHIMICA ACTA | 1990年 / 234卷 / 01期
关键词：
D O I：
10.1016/S0003-2670(00)83559-X
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The combination of immobilized alcohol oxidase (AOD) with a gas-diffusion membrane in a flow-injection system greatly enhanced the specificity of ethanol determination. This gas-diffusion flow-injection system with amperometric detection of hydrogen peroxide had a linear range of analysis from 0.0006 up to 60% (v/v) ethanol. Silicone-modified polypropylene membranes of different thickness were used. Undiluted samples of beer, wine, spirits and medicine could be investigated with excellent correlation with standard methods (r > 0.997). The frequency of analysis was 120-180 samples h-1 and the operational half-life of the immobilized enzyme was 8000 injections within 44 h. © 1990.
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页码：213 / 220
页数：8
相关论文
共 31 条
[1]   ENZYMATIC METHODS FOR THE DETERMINATION OF ETHANOL BASED ON LINEAR AND CYCLIC FLOW-INJECTION SYSTEMS [J].
ALMUAIBED, AM ;
TOWNSHEND, A .
ANALYTICA CHIMICA ACTA, 1988, 214 (1-2) :161-172
[2]  
BAADENHUISEN H, 1979, CLIN CHEM, V25, P433
[3]  
DRAWERT F, 1987, BRAUEREITECHNISCHE A, V2
[4]   VOLTAMMETRIC-ENZYMATIC DETERMINATION OF ETHANOL IN WHOLE-BLOOD BY FLOW-INJECTION ANALYSIS [J].
FERNANDEZ, A ;
DECASTRO, MDL ;
VALCARCEL, M .
FRESENIUS ZEITSCHRIFT FUR ANALYTISCHE CHEMIE, 1987, 327 (5-6) :552-554
[5]   HYDRODYNAMICALLY LIMITED PRECISION OF GRADIENT TECHNIQUES IN FLOW-INJECTION ANALYSIS [J].
GISIN, M ;
THOMMEN, C ;
MANSFIELD, KF .
ANALYTICA CHIMICA ACTA, 1986, 179 :149-167
[6]   AUTOMATIC ENZYMATIC-FLUORIMETRIC DETERMINATION OF ETHANOL IN BLOOD BY FLOW-INJECTION ANALYSIS [J].
GOMEZ, AF ;
POLONIO, JR ;
DECASTRO, L ;
CASES, MV .
CLINICA CHIMICA ACTA, 1985, 148 (02) :131-137
[7]  
Gulberg E L, 1980, J Automat Chem, V2, P189, DOI 10.1155/S1463924680000570
[8]   AMPEROMETRIC DETECTION OF SIMPLE ALCOHOLS IN AQUEOUS-SOLUTIONS BY APPLICATION OF A TRIPLE-PULSE POTENTIAL WAVEFORM AT PLATINUM-ELECTRODES [J].
HUGHES, S ;
MESCHI, PL ;
JOHNSON, DC .
ANALYTICA CHIMICA ACTA, 1981, 132 (DEC) :1-10
[9]  
IMATO T, 1988, BUNSEKI KAGAKU, V37, pT105
[10]  
KOJIMA T, 1983, BUNSEKI KAGAKU, V32, pE101
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