IDENTIFICATION OF 2 FAMILIES OF SATELLITE-LIKE REPETITIVE DNA-SEQUENCES FROM THE ZEBRAFISH (BRACHYDANIO-RERIO)

被引:35
作者
EKKER, M [1 ]
FRITZ, A [1 ]
WESTERFIELD, M [1 ]
机构
[1] UNIV OREGON,INST NEUROSCI,EUGENE,OR 97403
关键词
D O I
10.1016/0888-7543(92)90033-O
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
To further our understanding of the structure and organization of the zebrafish genome, we have undertaken the analysis of highly and middle-repetitive DNA sequences. We have cloned and sequenced two families of tandemly repeated DNA fragments. The monomer units of the Type I satellite-like sequence are 186 bp long, A+T-rich (65%), and exhibit a high degree of sequence conservation. The Type I satellite-like sequence constitutes 8% of the zebrafish genome, or approximately 8 × 105 copies per haploid genome. Southern analysis of genomic DNA, digested with several restriction endonucleases, shows a ladder of hybridizing bands, consistent with a tandem array, and suggests longer range periodic variations in the sequence of the tandem repeats. The Type II satellite has a monomer length of 165 bp, is also A+T-rich (68%), and constitues 0.2% of the zebrafish genome (22,000 copies per haploid genome). Southern analysis reveals a complex pattern rather than a ladder of regularly spaced hybridizing bands. © 1992.
引用
收藏
页码:1169 / 1173
页数:5
相关论文
共 28 条
[1]   MOLECULAR ARRANGEMENT AND EVOLUTION OF HETEROCHROMATIC DNA [J].
BRUTLAG, DL .
ANNUAL REVIEW OF GENETICS, 1980, 14 :121-144
[2]   CLONING AND CHARACTERIZATION OF A HIGHLY REPETITIVE FISH NUCLEOTIDE-SEQUENCE [J].
DATTA, U ;
DUTTA, P ;
MANDAL, RK .
GENE, 1988, 62 (02) :331-336
[3]   THE RECENT EVOLUTION OF MAMMALIAN REPETITIVE DNA ELEMENTS [J].
DEININGER, PL ;
DANIELS, GR .
TRENDS IN GENETICS, 1986, 2 (03) :76-80
[4]   A SATELLITE DNA FAMILY FROM POLLOCK (POLLACHIUS-VIRENS) [J].
DENOVAN, EM ;
WRIGHT, JM .
GENE, 1990, 87 (02) :279-283
[5]  
Franck J.P.C., 1991, Oxford Surveys on Eukaryotic Genes, V7, P51
[6]   CYTOGENETIC STUDIES IN NORTH-AMERICAN MINNOWS (CYPRINIDAE) .10. CHROMOSOMAL HETEROCHROMATIN DIFFERENTIATION IN NORTH-AMERICAN CYPRINID FISHES [J].
GOLD, JR ;
AMEMIYA, CT ;
ELLISON, JR .
CYTOLOGIA, 1986, 51 (03) :557-566
[7]   EVOLUTION OF CELLULAR DNA CONTENT IN TELEOST FISHES [J].
HINEGARD.R .
AMERICAN NATURALIST, 1968, 102 (928) :517-&
[8]   AN ANALYSIS OF FISH GENOMES BY DENSITY GRADIENT CENTRIFUGATION [J].
HUDSON, AP ;
CUNY, G ;
CORTADAS, J ;
HASCHEMEYER, AEV ;
BERNARDI, G .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1980, 112 (02) :203-210
[9]   SHORT INTERSPERSED REPETITIVE DNA ELEMENTS IN EUKARYOTES - TRANSPOSABLE DNA ELEMENTS GENERATED BY REVERSE TRANSCRIPTION OF RNA POL-III TRANSCRIPTS [J].
JAGADEESWARAN, P ;
FORGET, BG ;
WEISSMAN, SM .
CELL, 1981, 26 (02) :141-142
[10]   REPETITIVE SEQUENCES IN EUKARYOTIC DNA AND THEIR EXPRESSION [J].
JELINEK, WR ;
SCHMID, CW .
ANNUAL REVIEW OF BIOCHEMISTRY, 1982, 51 :813-844